Aaly-Gharibeh Zahra, Hosseinchi Mohammadreza, Shalizar-Jalali Ali
DVM Graduate, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran.
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran.
Vet Res Forum. 2024;15(2):113-117. doi: 10.30466/vrf.2023.2006604.3935. Epub 2024 Feb 15.
The precise pathophysiology of polycystic ovary syndrome (PCOS) is not well-founded. In an attempt to fill this gap, the current study was executed to probe the effect of nanocurcumin (NCC) on ovarian tissue, fertilization (IVF) and pre-implantation embryo development in a mouse model of PCOS. Fifty adult female mice were randomly categorized into five equal groups including non-treated control and PCOS (receiving 0.20 mg estradiol valerate (EV) intra-peritoneally once a day for 21 days) as well as NCC + PCOS, NCC + PCOS and NCC + PCOS (receiving respectively 12.50, 25.00 and 50.00 mg kg NCC daily along with EV injection through oral gavages for 21 days) groups. Subsequently, ovarian histo-architecture and total anti-oxidant capacity, and malonaldehyde and catalase levels as well as fertilizing potential, early embryonic development and serum testosterone concentration were analyzed. Results showed that NCC in a dose-dependent manner improved ovarian cyto-architectural organization and oxidant/anti-oxidant balance along with IVF rate and pre-implantation embryo development in PCOS mice. These findings revealed that NCC at the doses of 25.00 and 50.00 mg kg could alleviate PCOS-linked reproductive disruptions in female mice.
多囊卵巢综合征（PCOS）的确切病理生理学尚无充分依据。为了填补这一空白，本研究旨在探讨纳米姜黄素（NCC）对PCOS小鼠模型卵巢组织、受精（IVF）及植入前胚胎发育的影响。将50只成年雌性小鼠随机分为五组，每组数量相等，包括未处理的对照组和PCOS组（每天腹腔注射0.20 mg戊酸雌二醇（EV），共21天），以及NCC + PCOS组、NCC + PCOS组和NCC + PCOS组（分别每天通过口服灌胃给予12.50、25.00和50.00 mg/kg NCC，并同时注射EV，共21天）。随后，分析卵巢组织学结构、总抗氧化能力、丙二醛和过氧化氢酶水平，以及受精潜力、早期胚胎发育和血清睾酮浓度。结果表明，NCC以剂量依赖的方式改善了PCOS小鼠的卵巢细胞结构组织和氧化/抗氧化平衡，以及IVF率和植入前胚胎发育。这些发现表明，25.00和50.00 mg/kg剂量的NCC可以减轻雌性小鼠PCOS相关的生殖功能障碍。
