工程化 3D 基因组组织。
Engineering 3D genome organization.
机构信息
Department of Bioengineering, Stanford University, Stanford, CA, USA.
Department of Chemical and Systems Biology, Stanford University, Stanford, CA, USA.
出版信息
Nat Rev Genet. 2021 Jun;22(6):343-360. doi: 10.1038/s41576-020-00325-5. Epub 2021 Feb 8.
Cancers and developmental disorders are associated with alterations in the 3D genome architecture in space and time (the fourth dimension). Mammalian 3D genome organization is complex and dynamic and plays an essential role in regulating gene expression and cellular function. To study the causal relationship between genome function and its spatio-temporal organization in the nucleus, new technologies for engineering and manipulating the 3D organization of the genome have been developed. In particular, CRISPR-Cas technologies allow programmable manipulation at specific genomic loci, enabling unparalleled opportunities in this emerging field of 3D genome engineering. We review advances in mammalian 3D genome engineering with a focus on recent manipulative technologies using CRISPR-Cas and related technologies.
癌症和发育障碍与空间和时间（第四维）中 3D 基因组结构的改变有关。哺乳动物的 3D 基因组组织复杂且动态，并在调节基因表达和细胞功能方面发挥着重要作用。为了研究基因组功能与其在核内的时空组织之间的因果关系，已经开发出用于工程设计和操纵基因组 3D 组织的新技术。特别是，CRISPR-Cas 技术可在特定基因组位点进行可编程操作，从而为 3D 基因组工程这一新兴领域带来了前所未有的机遇。我们综述了哺乳动物 3D 基因组工程的进展，重点介绍了使用 CRISPR-Cas 和相关技术的最新操纵技术。
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