Acta Chim Slov. 2020 Mar;67(1):235-245.
The present work reveals the influence of copper (Cu) doping in band gap energy level as well as selective cytotoxicity of ZnO nanoparticles against human breast cancer cells (MCF7), human cervical carcinoma (HeLa), and one normal (Vero) cell line. Cu-doped ZnO nanoparticles (Cu-ZnO NPs) were synthesized and validated by UV-Vis, FT-IR, XRD, SEM and EDX. Cu-doping diminished the band gap energy of ZnO NPs from 3.54 eV to 3.29 eV. Antimicrobial activity has been evaluated against three bacterial and fungal strains. Antioxidant activity was examined using a DPPH free radical, ABTS+ radicals, hydroxyl radicals and nitric oxide scavenging assay. Cu-ZnO NPs showed anticancer activity with IC50 value of 219.56 µg/mL against MCF7 and 137.27 µg/mL against HeLa cell lines. The doping of Cu with ZnO improved the selective cytotoxicity of ZnO NPs towards MCF7 and HeLa cells without affecting the normal cells.
本研究揭示了铜（Cu）掺杂对氧化锌纳米粒子带隙能级的影响，以及其对人乳腺癌细胞（MCF7）、人宫颈癌细胞（HeLa）和一种正常细胞系（Vero）的选择性细胞毒性。通过紫外-可见分光光度计（UV-Vis）、傅里叶变换红外光谱（FT-IR）、X 射线衍射（XRD）、扫描电子显微镜（SEM）和能谱（EDX）对 Cu 掺杂氧化锌纳米粒子（Cu-ZnO NPs）进行了合成和验证。Cu 掺杂使 ZnO NPs 的带隙能从 3.54eV 降低到 3.29eV。对三种细菌和真菌菌株进行了抗菌活性评估。采用 DPPH 自由基、ABTS+自由基、羟基自由基和一氧化氮清除实验检测了抗氧化活性。Cu-ZnO NPs 对 MCF7 的 IC50 值为 219.56μg/mL，对 HeLa 的 IC50 值为 137.27μg/mL，表现出抗肿瘤活性。Cu 掺杂 ZnO 提高了 ZnO NPs 对 MCF7 和 HeLa 细胞的选择性细胞毒性，而对正常细胞没有影响。
