基于全基因组鉴定、分析与表达的紫菀木 TIFY 转录因子研究

Genome-wide investigation of the TIFY transcription factors in alfalfa (Medicago sativa L.): identification, analysis, and expression.

机构信息

Technology Engineering Center of Drought and Cold-Resistant Grass Breeding in North of the National Forestry and Grassland Administration, College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot, China.

Key Laboratory of Grassland Resources of the Ministry of Education, College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot, China.

出版信息

BMC Plant Biol. 2024 Sep 6;24(1):840. doi: 10.1186/s12870-024-05378-w.

DOI:10.1186/s12870-024-05378-w

PMID:39242996

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11378388/

Abstract

BACKGROUND

Alfalfa (Medicago sativa L.) is an essential leguminous forage with high nutrition and strong adaptability. The TIFY family is a plant-specific transcription factor identified in many plants. However, few reports have been reported on the phylogenetic analysis and gene expression profiling of TIFY family genes in alfalfa.

RESULT

A total of 84 TIFY genes belonging to 4 categories were identified in alfalfa, including 58 MsJAZs, 18 MsZMLs, 4 MsTIFYs and 4 MsPPDs, respectively. qRT-PCR data from 8 genes in different tissues revealed that most MsTIFY genes were highly expressed in roots. The expression of MsTIFY14 was up-regulated after different times in both thrips-resistant and susceptible alfalfa after thrips feeding, and the expression of the remaining MsTIFYs had a strong correlation with the time of thrips feeding. Different abiotic stresses, including drought, salt, and cold, could induce or inhibit the expression of MsTIFY genes to varying degrees. In addition, the eight genes were all significantly up-regulated by JA and/or SA. Interestingly, MsTIFY77 was induced considerably by all the biotic, abiotic, or plant hormones (JA or SA) except ABA.

CONCLUSION

Our study identified members of the TIFY gene family in alfalfa and analyzed their structures and possible functions. It laid the foundation for further research on the molecular functions of TIFYs in alfalfa.

摘要

背景

紫花苜蓿（Medicago sativa L.）是一种重要的豆科饲用植物，具有高营养价值和强适应性。TIFY 家族是在许多植物中发现的一种植物特异性转录因子。然而，关于苜蓿 TIFY 家族基因的系统发育分析和基因表达谱的报道较少。

结果

在苜蓿中鉴定出 84 个 TIFY 基因，分为 4 类，包括 58 个 MsJAZs、18 个 MsZMLs、4 个 MsTIFYs 和 4 个 MsPPDs。8 个基因在不同组织中的 qRT-PCR 数据显示，大多数 MsTIFY 基因在根中高度表达。在受到蓟马侵害和易感苜蓿后不同时间的蓟马取食后，MsTIFY14 的表达上调，其余 MsTIFYs 的表达与蓟马取食时间密切相关。不同的非生物胁迫，包括干旱、盐和寒冷，可不同程度地诱导或抑制 MsTIFY 基因的表达。此外，这 8 个基因都被 JA 和/或 SA 显著上调。有趣的是，除了 ABA 外，MsTIFY77 被所有生物、非生物或植物激素（JA 或 SA）显著诱导。