College of Food Science and Engineering, South China University of Technology, Guangzhou, Guangdong, 510640, China.
College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, Guangdong, 510006, China.
J Food Sci. 2021 Mar;86(3):977-986. doi: 10.1111/1750-3841.15605. Epub 2021 Feb 8.
In this study, the response surface method was employed to optimize the extraction conditions of the ultrasonic-assisted extraction of Sargassum fusiforme polysaccharides (SFPS). The effects of four independent variables (hot water extraction time, ultrasonic time, ultrasonic power, and material-to-liquid ratio) on the extraction rate of SFPS were tested. In addition, the SFPS functionalized nanoselenium (SFPS-SeNPs) was prepared by chemical reduction method, whose characterization and in vitro antioxidant activity were investigated. The results showed that the yield of the crude SFPS was 25.8% at the optimal conditions of material-to-liquid ratio 1:50 (w/v), ultrasonic power 200 W, ultrasonic time 15 min, and water bath time 130 min. A series of characterization experiments showed that the SFPS-SeNPs performed higher dispersion and stability than naked SeNPs. Furthermore, the in vitro antioxidant activity assay indicated that SFPS functioned as a modifier improved the free radical scavenging activity of SeNPs significantly. In conclusion, this study provided a method to extract SFPS as a carrier for SeNPs, and SFPS-SeNPs could not only improve the stability of SeNPs, but also exerted the biological activities of SFPS. PRACTICAL APPLICATION: This research provided new ideas for the application of SFPS and the development of nanoselenium preparation carriers.
在这项研究中，采用响应面法优化了超声辅助提取羊栖菜多糖（SFPS）的提取条件。测试了四个独立变量（热水提取时间、超声时间、超声功率和固液比）对 SFPS 提取率的影响。此外，通过化学还原法制备了 SFPS 功能化纳米硒（SFPS-SeNPs），并对其进行了表征和体外抗氧化活性研究。结果表明，在固液比 1:50（w/v）、超声功率 200 W、超声时间 15 min 和水浴时间 130 min 的最佳条件下，粗 SFPS 的产率为 25.8%。一系列表征实验表明，SFPS-SeNPs 比裸露的 SeNPs 具有更高的分散性和稳定性。此外，体外抗氧化活性测定表明，SFPS 作为修饰剂显著提高了 SeNPs 的自由基清除活性。总之，本研究为 SFPS 作为 SeNPs 载体的提取提供了一种方法，SFPS-SeNPs 不仅可以提高 SeNPs 的稳定性，还能发挥 SFPS 的生物活性。实际应用：本研究为 SFPS 的应用和纳米硒制备载体的开发提供了新思路。
