PhD Program in Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
Nutrients. 2023 Feb 17;15(4):1018. doi: 10.3390/nu15041018.
is an orchid that contains phenolic compounds and exhibits antioxidant and anti-inflammation properties. This study aimed to investigate whether its ethanolic extract (ABE) can be used as a reducing agent and/or a stabilizer of nano-selenium (SeNP) synthesis. SeNPs exhibited higher antioxidant activity than ABE-SeNPs. In contrast, ABE-SeNP (4 µM Se) had greater anti-inflammatory activity in LPS-induced macrophages than SeNPs. Interestingly, ABE acted as a stabilizer for SeNPs by preventing particle aggregation and preserving its antioxidant activity after long-term storage (90 days). Moreover, after the freeze-drying process, ABE-SeNPs could be completely reconstituted to suspension with significantly stable antioxidant and anti-inflammatory activities compared to freshly prepared particles, suggesting the cryoprotectant and/or lyoprotectant role of ABE. The present study shows the potential of ABE as an effective stabilizer for nanoparticles and provides evidence for the development of ABE-SeNPs as a food supplement or novel functional ingredient for health benefits.
铁皮石斛是一种含有酚类化合物的兰花，具有抗氧化和抗炎特性。本研究旨在探讨其乙醇提取物（ABE）是否可用作纳米硒（SeNP）合成的还原剂和/或稳定剂。SeNPs 表现出比 ABE-SeNPs 更高的抗氧化活性。相比之下，ABE-SeNP（4 µM Se）在 LPS 诱导的巨噬细胞中具有比 SeNPs 更强的抗炎活性。有趣的是，ABE 通过防止颗粒聚集并在长期储存（90 天）后保持其抗氧化活性来充当 SeNPs 的稳定剂。此外，经过冷冻干燥过程后，ABE-SeNPs 可以完全重新配制为悬浮液，与新制备的颗粒相比，具有显著稳定的抗氧化和抗炎活性，这表明 ABE 具有冷冻保护剂和/或冻干保护剂的作用。本研究表明 ABE 作为纳米颗粒有效稳定剂的潜力，并为开发 ABE-SeNPs 作为食品补充剂或新型功能性成分以促进健康提供了证据。
