Shanmugam Rajeshkumar, Anandan Jayasree, Balasubramanian Ashwin K, Raja Rupa D, Ranjeet Srivarsha, Deenadayalan Pavithra
Department of Pharmacology, Saveetha Dental College and Hospital, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, IND.
Department of Pharmacology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, IND.
Cureus. 2023 Dec 20;15(12):e50861. doi: 10.7759/cureus.50861. eCollection 2023 Dec.
Background Antioxidants are vital in reducing oxidative stress, a key factor in the pathogenesis of many chronic diseases, including cancer, cardiovascular disease, and neurodegenerative disorders. The aim of our study is to analyze and compare the oxidative potential of biosynthesized selenium, strontium, and zinc oxide nanoparticles (NPs). Materials and methods Selenium nanoparticles (SeNPs) were synthesized using 20 mM of sodium selenite as the precursor and 1 g each of and as reducing and stabilizing agents. Strontium nanoparticles (SrNPs) were synthesized with 30 mM of strontium chloride as the precursor and 1 g of as a reducing and stabilizing agent. Zinc oxide nanoparticles (ZnONPs) were synthesized using 30 mM of zinc nitrate as the precursor and 1 g each of and as reducing and stabilizing agents. Selenium, strontium, and zinc oxide nanoparticles were characterized using Fourier-transform infrared spectroscopy (FT-IR) analysis. The antioxidant activity of biogenically synthesized strontium, selenium and zinc oxide nanoparticles was examined using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay (DPPH assay) and hydroxyl radical scavenging assay (HO assay). Results The FT-IR spectra of selenium nanoparticles revealed a peak at 3327.990 cm, strontium nanoparticles at 3332.331 cm, and zinc oxide nanoparticles at 3216.346 cm. The significant results for the green-synthesized selenium, strontium, and zinc oxide nanoparticles were observed in antioxidant assays. The results from the DPPH assay show that at the highest concentration of 50 µL, SrNPs exhibited 90.12 % inhibition, SeNPs displayed 90.12% inhibition, and ZnONPs showed 89.55% inhibition. In the HO assay, at the highest concentration of 50 µL, SrNPs showed 87.43% inhibition, SeNPs displayed 85.11% inhibition, and ZnONPs exhibited 84.66% inhibition. SrNPs demonstrated a higher percentage of inhibition in both the DPPH and HO assays. Maximum inhibitory activity was observed at the highest concentration. However, the prepared nanoparticles showed a slightly lower percentage of inhibition when compared to the standard. Conclusion Strontium nanoparticles synthesized based on demonstrated excellent antioxidant activity compared to the synthesized selenium and zinc oxide nanoparticles. Therefore, the study suggests that the produced strontium nanoparticles can serve as an antioxidant agent, owing to their remarkable free radical scavenging activity.
背景 抗氧化剂在减轻氧化应激方面至关重要，氧化应激是包括癌症、心血管疾病和神经退行性疾病在内的许多慢性疾病发病机制中的关键因素。我们研究的目的是分析和比较生物合成的硒、锶和氧化锌纳米颗粒（NPs）的氧化潜力。
材料与方法 以20 mM亚硒酸钠为前驱体，分别以1 g 和 作为还原剂和稳定剂合成硒纳米颗粒（SeNPs）。以30 mM氯化锶为前驱体，1 g 作为还原剂和稳定剂合成锶纳米颗粒（SrNPs）。以30 mM硝酸锌为前驱体，分别以1 g 和 作为还原剂和稳定剂合成氧化锌纳米颗粒（ZnONPs）。使用傅里叶变换红外光谱（FT-IR）分析对硒、锶和氧化锌纳米颗粒进行表征。使用2,2-二苯基-1-苦基肼（DPPH）自由基清除试验（DPPH试验）和羟基自由基清除试验（HO试验）检测生物合成的锶、硒和氧化锌纳米颗粒的抗氧化活性。
结果 硒纳米颗粒的FT-IR光谱在3327.990 cm处有一个峰，锶纳米颗粒在3332.331 cm处，氧化锌纳米颗粒在3216.346 cm处。在抗氧化试验中观察到绿色合成的硒、锶和氧化锌纳米颗粒有显著结果。DPPH试验结果表明，在最高浓度50 μL时，SrNPs的抑制率为90.12%，SeNPs为90.12%，ZnONPs为89.55%。在HO试验中，在最高浓度50 μL时，SrNPs的抑制率为87.43%，SeNPs为85.11%，ZnONPs为84.66%。SrNPs在DPPH和HO试验中均表现出较高的抑制率。在最高浓度下观察到最大抑制活性。然而，与标准品相比，制备的纳米颗粒的抑制率略低。
结论 基于 合成的锶纳米颗粒与合成的硒和氧化锌纳米颗粒相比，具有优异的抗氧化活性。因此，该研究表明，所制备的锶纳米颗粒因其显著的自由基清除活性可作为一种抗氧化剂。
