Department of Entomology, China Agricultural University, Beijing 100193, China.
Department of Entomology, China Agricultural University, Beijing 100193, China.
Ecotoxicol Environ Saf. 2021 Apr 1;212:111969. doi: 10.1016/j.ecoenv.2021.111969. Epub 2021 Feb 6.
Sublethal effect considered as an emerging factor to assess the environmental risk of insecticides, which can impact the insects on both physiology and behavior. Lethal exposure can be causing near immediate mortality. Pests are inevitably exposed to sublethal and lethal dose in the agroecosystem following application of pesticides. Insecticides, widely used for the control of insect pests, are irreplaceable in insect pest management. The effects of imidacloprid by the method of high-throughput non-targeted metabolomics was investigated in Aphis gossypii Glover exposed to LC and LC doses of the imidacloprid, and the control group was treated with the same condition without imidacloprid. Pairwise comparisons showed that 111 metabolites changed significantly, 60 in the LC group, and 66 in the LC group compared to the control group, while only 16 changes in the LC were same with that in LC group. Among the changed metabolites, a total of 16 metabolites were identified as potential biomarkers, which represented the most influential pathways including glycolysis and gluconeogenesis, alanine, aspartate, and glutamate metabolism, ascorbate and aldarate metabolism, glutathione metabolism, phenylalanine metabolism, tyrosine metabolism, caffeine metabolism and parkinson's disease (PD), which could account for the sublethal and lethal effects on A. gossypii. These modified metabolic pathways demonstrated that high energy consumption, excitotoxicity and oxidative stress (OS) were appeared in both LC and LC groups, while PD was detected only in the LC group. The results of non-targeted metabolomics revealed the effects of neonicotinoid pesticide exposure on A. gossypii successfully, and provided a deep insight into the influenced physiology by the stress of neonicotinoid pesticide in the insect.
亚致死效应被认为是评估杀虫剂环境风险的一个新出现的因素，它会对昆虫的生理和行为产生影响。致死暴露会导致立即死亡。在应用农药后，害虫不可避免地会在农业生态系统中接触到亚致死和致死剂量。杀虫剂被广泛用于防治害虫，在害虫管理中是不可替代的。本研究采用高通量非靶向代谢组学方法，研究了噻虫啉对棉蚜的影响，LC 和 LC 剂量的噻虫啉暴露组和对照组的处理条件相同。成对比较表明，与对照组相比，LC 组有 60 种代谢物和 LC 组有 66 种代谢物发生显著变化，而 LC 组与 LC 组相同的变化只有 16 种。在变化的代谢物中，共有 16 种代谢物被鉴定为潜在的生物标志物，它们代表了最有影响的途径，包括糖酵解和糖异生、丙氨酸、天冬氨酸和谷氨酸代谢、抗坏血酸和醛酸代谢、谷胱甘肽代谢、苯丙氨酸代谢、酪氨酸代谢、咖啡因代谢和帕金森病（PD），这些途径可以解释噻虫啉对棉蚜的亚致死和致死作用。这些改变的代谢途径表明，LC 和 LC 组都出现了高能量消耗、兴奋毒性和氧化应激（OS），而 PD 仅在 LC 组中检测到。非靶向代谢组学的结果成功地揭示了新烟碱类农药暴露对棉蚜的影响，深入了解了昆虫中新型烟碱类农药应激对其生理的影响。
