Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, College of Agriculture, Yangzhou University, Yangzhou 225009, China.
Co-Innovation Center for Modern Production Technology of Grain Crops of Jiangsu Province/Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education, Yangzhou University, Yangzhou 225009, China.
Int J Mol Sci. 2021 Feb 7;22(4):1661. doi: 10.3390/ijms22041661.
Seed dormancy and germination are key events in plant development and are critical for crop production, and defects in seed germination or the inappropriate release of seed dormancy cause substantial losses in crop yields. Rice is the staple food for more than half of the world's population, and preharvest sprouting (PHS) is one of the most severe problems in rice production, due to a low level of seed dormancy, especially under warm and damp conditions. Therefore, PHS leads to yield loss and a decrease in rice quality and vitality. We reveal that mutation of inhibited rice PHS. Analysis of the expression of and its encoded protein sequence and structure indicated that OsbZIP09 is a typical bZIP transcription factor that contains conserved bZIP domains, and its expression is induced by ABA. Moreover, RNA sequencing (RNA-seq) and DNA affinity purification sequencing (DAP-seq) analyses were performed and 52 key direct targets of OsbZIP09 were identified, including and () family genes, which are involved in controlling seed germination. Most of these key targets showed consistent changes in expression in response to abscisic acid (ABA) treatment and mutation. The data characterize a number of key target genes that are directly regulated by OsbZIP09 and contribute to revealing the molecular mechanism that underlies how OsbZIP09 controls rice seed germination.
种子休眠和萌发是植物发育的关键事件，对作物生产至关重要，种子萌发缺陷或种子休眠的不当释放会导致作物产量的大量损失。水稻是世界上一半以上人口的主食，而发芽是水稻生产中最严重的问题之一，这是由于种子休眠程度低，尤其是在温暖潮湿的条件下。因此，发芽会导致产量损失以及稻米质量和活力下降。我们揭示了 突变抑制了水稻的发芽。对 和其编码蛋白序列和结构的表达分析表明，OsbZIP09 是一种典型的 bZIP 转录因子，含有保守的 bZIP 结构域，其表达受 ABA 诱导。此外，还进行了 RNA 测序 (RNA-seq) 和 DNA 亲和纯化测序 (DAP-seq) 分析，鉴定了 52 个 OsbZIP09 的关键直接靶标，包括 和 ()家族基因，它们参与控制种子萌发。这些关键靶标中的大多数在响应脱落酸 (ABA)处理和 突变时表现出一致的表达变化。这些数据描绘了一系列直接受 OsbZIP09 调控的关键靶基因，有助于揭示 OsbZIP09 控制水稻种子萌发的分子机制。
