Koneru Meghana, Sahu Bidya Dhar, Gudem Sagarika, Kuncha Madhusudana, Ravuri Halley Gora, Kumar Jerald Mahesh, Kilari Eswar Kumar, Sistla Ramakrishna
Medicinal Chemistry and Pharmacology Division, CSIR-Indian Institute of Chemical Technology (IICT), Hyderabad-500 007, India.
Animal House Facility, CSIR-Centre for Cellular and Molecular Biology (CCMB), Hyderabad 500 007, India.
Phytomedicine. 2017 Apr 15;27:23-32. doi: 10.1016/j.phymed.2017.01.013. Epub 2017 Jan 31.
Alcohol, a most commonly consumed beverage, is the foremost cause of liver injury throughout the world. Polydatin, a stilbenoid glucoside, was known to possess antioxidant and anti-inflammatory properties and is being investigated for use in various disorders.
The present study was intended at investigating the hepatoprotective efficacy of polydatin against acute-alcohol induced liver injury model in mice.
C57BL/6 mice were fed with five doses of 50% ethyl alcohol (10ml/kg body weight) to induce acute liver injury. Effect of polydatin against alcohol induced hepatic injury was investigated by giving 50 or 100mg/kg polydatin, orally, for 8 days.
Serum markers of liver injury, morphology, histology and fibrosis of liver tissue, levels of enzymatic and non-enzymatic antioxidants and the mitochondrial respiratory enzyme activities in liver tissue were investigated. The activities and the protein expression of matrix metalloproteinases (MMP-2 and -9), the expression of NF-κB in the liver tissue were also studied.
Polydatin pre-treatment significantly alleviated the alcohol induced hepatic injury by reducing the serum liver injury markers, alanine aminotransferase (ALT) and aspartate aminotransferase (AST), attenuating oxidative stress and restoring antioxidant balance in the hepatic tissue. Simultaneously, polydatin pre-treatment also prevented alcohol induced mitochondrial damage and refurbished the matrix metalloproteinases levels of the hepatic tissue.
The findings of the present study suggest that polydatin may have a potential benefit in preventing alcohol-induced acute hepatic injury.
酒精是最常饮用的饮品，是全球肝损伤的首要原因。白藜芦醇苷是一种芪类糖苷，已知具有抗氧化和抗炎特性，目前正在对其在各种疾病中的应用进行研究。
本研究旨在探讨白藜芦醇苷对小鼠急性酒精性肝损伤模型的肝保护作用。
给C57BL/6小鼠喂食五剂50%乙醇（10毫升/千克体重）以诱导急性肝损伤。通过口服给予50或100毫克/千克白藜芦醇苷，持续8天，研究白藜芦醇苷对酒精性肝损伤的影响。
检测肝损伤血清标志物、肝脏组织形态学、组织学和纤维化、肝组织中酶促和非酶促抗氧化剂水平以及线粒体呼吸酶活性。还研究了基质金属蛋白酶（MMP-2和-9）的活性和蛋白表达、肝组织中NF-κB的表达。
白藜芦醇苷预处理通过降低血清肝损伤标志物谷丙转氨酶（ALT）和谷草转氨酶（AST），减轻氧化应激并恢复肝组织中的抗氧化平衡，显著减轻了酒精诱导的肝损伤。同时，白藜芦醇苷预处理还预防了酒精诱导的线粒体损伤，并恢复了肝组织中基质金属蛋白酶的水平。
本研究结果表明，白藜芦醇苷在预防酒精性急性肝损伤方面可能具有潜在益处。
