尼麦角林在糖尿病大鼠角膜伤口愈合中的作用。
Role of nicergoline in corneal wound healing in diabetic rats.
机构信息
Federal University of Pernambuco (UFPE), Recife, Pernambuco, Brazil.
Keizo-Asami Immunopathology Laboratory (LIKA), Recife, Pernambuco, Brazil.
出版信息
BMC Ophthalmol. 2021 Feb 9;21(1):77. doi: 10.1186/s12886-021-01835-4.
BACKGROUND
To investigate the effect of nicergoline on the rate of complete corneal ulcer reepithelialization (CCUR) in diabetic rats with diabetic keratopathy.
METHODS
Forty-eight streptozotocin-induced diabetic rats were randomly divided into two groups. The experimental group (n = 24) received nicergoline (10 mg.kg.day), while the control group (n = 24) received a placebo. A corneal epithelial defect was induced using a corneal diamond burr, and defect area was compared at time points of 0, 12, 24, 48 and 72 h after the injury using image analysis software. The probability of CCUR within 72 h was assessed using the Kaplan-Meier survival analysis log-rank test.
RESULTS
When compared, 4 of the 24 rats (17%) in the placebo group and 12 of the 24 rats (50%) in the nicergoline group were found to have CCUR within 72 h (log-rank = 0.027). Cox regression analysis found no effect of the covariates blood glucose (P = 0.601) or weight (P = 0.322) on the corneal reepithelialization (survival) curve.
CONCLUSIONS
Nicergoline increased wound healing rates relative to placebo and may therefore be investigated as a treatment option in diabetic keratopathy.
背景
研究尼麦角林对糖尿病角膜病变糖尿病大鼠完全角膜溃疡再上皮化（CCUR）率的影响。
方法
48 只链脲佐菌素诱导的糖尿病大鼠随机分为两组。实验组（n=24）给予尼麦角林（10mg.kg.day），对照组（n=24）给予安慰剂。使用角膜钻石钻头诱导角膜上皮缺损，并用图像分析软件比较损伤后 0、12、24、48 和 72 小时的缺损面积。采用 Kaplan-Meier 生存分析对数秩检验评估 72 小时内 CCUR 的概率。
结果
与安慰剂组相比，24 只大鼠中有 4 只（17%）和尼麦角林组中有 24 只大鼠中的 12 只（50%）在 72 小时内出现 CCUR（对数秩=0.027）。Cox 回归分析发现血糖（P=0.601）或体重（P=0.322）对角膜再上皮化（生存）曲线无影响。
结论
尼麦角林与安慰剂相比，增加了伤口愈合率，因此可能被研究作为糖尿病角膜病变的一种治疗选择。
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