Laboratório Nacional de Energia e Geologia, I.P., Unidade de Bioenergia, Estrada do Paço do Lumiar 22, 1649-038 Lisboa, Portugal.
Laboratório Nacional de Energia e Geologia, I.P., Unidade de Bioenergia, Estrada do Paço do Lumiar 22, 1649-038 Lisboa, Portugal.
Bioresour Technol. 2014 Apr;158:355-9. doi: 10.1016/j.biortech.2014.02.071. Epub 2014 Feb 28.
The present work studied low-cost carbon sources for carotenoid and lipid production using the yeast Rhodosporidum toruloides NCYC 921. Carob pulp syrup and sugarcane molasses at different concentrations were used as low-cost carbon sources in R. toruloides batch cultivations. Carob pulp syrup containing a total sugar concentration of 75 g L(-1) induced the highest total fatty acid productivity (1.90 g L(-1)h(-1)) and the highest carotenoid productivity (9.79 μg L(-1)h(-1)). Flow cytometric analysis revealed that most of the yeast cells (>60%) grown on carob pulp syrup displayed intact polarised membranes, conversely to the cells grown on sugarcane molasses, wherein a large proportion (>45%) displayed permeabilised cytoplasmic membranes.
本工作研究了使用酵母红酵母 NCYC 921 生产类胡萝卜素和脂质的低成本碳源。在红酵母分批培养中，使用不同浓度的角豆浆和甘蔗蜜饯作为低成本碳源。含有总糖浓度为 75 g L(-1)的角豆浆诱导产生的总脂肪酸生产率最高（1.90 g L(-1)h(-1)），类胡萝卜素生产率最高（9.79 μg L(-1)h(-1)）。流式细胞术分析表明，在角豆浆上生长的大多数酵母细胞（>60%）显示出完整的极性细胞膜，与在甘蔗蜜饯上生长的细胞相反，其中很大一部分（>45%）显示出细胞质膜通透性。
