伤寒杆菌Ty2的一个缺失Vi抗原的galE变异菌株在人体中仍具毒力。
A galE via (Vi antigen-negative) mutant of Salmonella typhi Ty2 retains virulence in humans.
作者信息
Hone D M, Attridge S R, Forrest B, Morona R, Daniels D, LaBrooy J T, Bartholomeusz R C, Shearman D J, Hackett J
机构信息
Department of Microbiology and Immunology, University of Adelaide, South Australia.
出版信息
Infect Immun. 1988 May;56(5):1326-33. doi: 10.1128/iai.56.5.1326-1333.1988.
Abstract
We have recently described the construction of a galE derivative of Salmonella typhi Ty2 (Ty2H1) which had a 0.4-kilobase deletion in the galE gene and was sensitive to galactose-induced lysis when cultured with greater than or equal to 0.06 mM galactose (D. M. Hone, R. Morona, S. Attridge, and J. Hackett, J. Infect. Dis. 156:167-174, 1987). We now report the selection of a rifampin-resistant, via derivative of Ty2H1, EX462. Compared with the Ty2 parent strain, EX462 was serum sensitive and highly attenuated in the mouse mucin virulence assay. When four human volunteers ingested 7 X 10(8) viable EX462, two became ill and developed a typhoidlike disease with fever and bacteremia. Blood isolates from these individuals were indistinguishable from the vaccine strain by a variety of criteria. We concluded that, even in a via background, the galE mutation was not attenuating for S. typhi in humans.
摘要
我们最近描述了伤寒杆菌Ty2（Ty2H1）的galE衍生物的构建，该衍生物在galE基因中有一个0.4千碱基的缺失，当在大于或等于0.06 mM半乳糖的条件下培养时，对半乳糖诱导的裂解敏感（D.M.霍恩、R.莫罗纳、S.阿特里奇和J.哈克特，《传染病杂志》156:167 - 174，1987年）。我们现在报告通过Ty2H1的衍生物EX462筛选出一种耐利福平的菌株。与Ty2亲本菌株相比，EX462对血清敏感，并且在小鼠粘蛋白毒力试验中高度减毒。当四名人类志愿者摄入7×10⁸个活的EX462时，两人患病并出现了类似伤寒的疾病，伴有发热和菌血症。通过多种标准，从这些个体血液中分离出的菌株与疫苗株无法区分。我们得出结论，即使在via背景下，galE突变对人类伤寒杆菌也没有减毒作用。
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