Formal S B, Baron L S, Kopecko D J, Washington O, Powell C, Life C A
Infect Immun. 1981 Dec;34(3):746-50. doi: 10.1128/iai.34.3.746-750.1981.
Shigella sonnei, an intestinal pathogen, produces a characteristic form I cell surface antigen now known to be plasmid encoded. We considered that the GalE Salmonella typhi Ty21a oral vaccine strain, highly effective against typhoid, might be modified so as to be protective also against shigellosis due to S. sonnei. The plasmid responsible for form I antigen synthesis was therefore conjugally transferred to the galE S. typhi strain. Serological studies revealed that the derivative strain produces the form I antigen in addition to the normal S. typhi somatic antigens. Testing in mice demonstrated that the derivative form I galE S. typhi strain is protective against both S. sonnei and S. typhi challenges. These data suggest that the galE S. Ty21a oral vaccine strain, which presumably stimulates the local immune system in the intestine, may also serve as a useful carrier for other antigenic determinants to protect against different intestinal infections.
宋内志贺菌是一种肠道病原体，可产生一种特征性的I型细胞表面抗原，现已知道该抗原由质粒编码。我们认为，对伤寒有高效预防作用的GalE伤寒沙门菌Ty21a口服疫苗株，或许可进行改造，使其也能预防宋内志贺菌引起的志贺菌病。因此，负责I型抗原合成的质粒通过接合转移至GalE伤寒沙门菌菌株。血清学研究显示，该衍生菌株除了产生正常的伤寒沙门菌菌体抗原外，还产生I型抗原。在小鼠身上进行的试验表明，衍生的I型GalE伤寒沙门菌菌株对宋内志贺菌和伤寒沙门菌的攻击均有保护作用。这些数据表明，GalE伤寒沙门菌Ty21a口服疫苗株大概能刺激肠道局部免疫系统，它或许还可作为其他抗原决定簇的有效载体，以预防不同的肠道感染。
