Johns Paul W, Hertzler Steven R
Abbott Laboratories, Abbott Nutrition Division, 3300 Stelzer Road, Columbus, OH, USA 43219.
Int J Food Sci. 2021 Jan 29;2021:6669544. doi: 10.1155/2021/6669544. eCollection 2021.
A commercial fava bean protein isolate and a liquid nutritional product formulated with it were tested by validated HPLC methods for the favism-associated pyrimidine glycoside vicine, the dopamine precursor levodopa, and the biogenic amine tyramine. The vicine, levodopa, and tyramine concentrations in the protein isolate-306, 13.3, and <0.5 mg/kg, respectively-when expressed on a protein basis-34, 1.5, and <0.06 mg/100 g protein, respectively-were at least 96% lower than the vicine, levodopa, and tyramine (protein-based) concentrations reported for fava beans (≥900, ~200, and ~4 mg/100 g protein, respectively). This was also true for the vicine (13 mg/kg or 22 mg/100 g protein), levodopa (≤0.17 mg/kg or ≤0.3 mg/100 g protein), and tyramine (0.08 mg/kg or 0.14 mg/100 g protein) concentrations in the nutritional product. On the basis of these data, one serving (11 fl. oz.) of the nutritional product would deliver approximately 5 mg of vicine, <1 mg of levodopa, and <0.1 mg of tyramine.
采用经过验证的高效液相色谱法，对一种商业化的蚕豆分离蛋白及其制成的液体营养产品进行检测，以测定与蚕豆病相关的嘧啶糖苷蚕豆嘧啶、多巴胺前体左旋多巴和生物胺酪胺的含量。以蛋白质计，分离蛋白中蚕豆嘧啶、左旋多巴和酪胺的浓度分别为306、13.3和<0.5mg/kg，即分别为34、1.5和<0.06mg/100g蛋白质，至少比蚕豆中报道的（分别为≥900、200和4mg/100g蛋白质）基于蛋白质的蚕豆嘧啶、左旋多巴和酪胺浓度低96%。营养产品中的蚕豆嘧啶（13mg/kg或22mg/100g蛋白质）、左旋多巴（≤0.17mg/kg或≤0.3mg/100g蛋白质）和酪胺（0.08mg/kg或0.14mg/100g蛋白质）浓度也是如此。根据这些数据，一份（11液量盎司）营养产品大约含有5mg蚕豆嘧啶、<1mg左旋多巴和<0.1mg酪胺。
