具有预防骨质疏松症功能的 - 衍生肽的吸收和转运。
Absorption and transport of a -derived peptide with the function of preventing osteoporosis.
机构信息
School of Food Science and Technology, National Engineering Research Center of Seafood, Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian 116034, China.
College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210046, China.
出版信息
Food Funct. 2021 Mar 15;12(5):2102-2111. doi: 10.1039/d0fo02353a.
The YPRKDETGAERT peptide (PME-1) identified from the Mytilus edulis proteins has been shown to promote the proliferation and differentiation of osteoblasts and it has good bone-forming activity in vitro. Further, PME-1 has been shown to prevent osteoporosis in vivo. PME-1 can be absorbed through the gastrointestinal tract, and the passing rate in monolayer Caco-2 cells was 6.57%. PME-1 can also enter the blood circulation and the concentration of PME-1 in serum reached the maximum, 61.06 ± 26.32 ng mL-1, 20 min after feeding. The multifunctional in vivo imager was used to further determine the distribution of the 5-FITC-(Acp)-YPRKDETGAERT peptide (PME-1-FITC) 2 h after feeding the peptide, and the result confirmed the above results and showed that a part of PME-1-FITC can affect bone in vivo. Therefore, PME-1 not only was easily absorbed in the gastrointestinal tract, but also has the potential beneficial effect on preventing osteoporosis.
从贻贝蛋白中鉴定出的 YPRKDETGAERT 肽（PME-1）已被证明能促进成骨细胞的增殖和分化，并具有良好的体外成骨活性。此外，PME-1 已被证明能预防体内骨质疏松症。PME-1 可以通过胃肠道吸收，在单层 Caco-2 细胞中的通过率为 6.57%。PME-1 也可以进入血液循环，在喂食 20 分钟后，血清中 PME-1 的浓度达到最大值，为 61.06±26.32ng/mL。多功能体内成像仪进一步确定了在喂食肽后 2 小时 5-FITC-(Acp)-YPRKDETGAERT 肽（PME-1-FITC）的分布，结果证实了上述结果，并表明 PME-1-FITC 的一部分可以在体内影响骨骼。因此，PME-1 不仅在胃肠道内易被吸收，而且对预防骨质疏松症具有潜在的有益作用。
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