Center for Clinical and Translational Research, Virginia Commonwealth University School of Medicine , Richmond, VA, USA.
Department of Microbiology and Immunology, Virginia Commonwealth University School of Medicine , Richmond, VA, USA.
Adipocyte. 2021 Dec;10(1):91-100. doi: 10.1080/21623945.2021.1880083.
The transgenic mouse is widely used in the development of adipocyte-specific genetic manipulations for the study of obesity and type 2 diabetes. In the process of developing a new mouse model utilizing the adipocyte selective transgenic mouse, strong genetic linkage between a gene of interest, , and the transgene was discovered. Whole-genome sequencing of the transgenic mouse model identified the genomic insertion site within the gene locus on chromosome 9 and this insertion causes a significant decrease in gene expression in adipose tissue. Insertion of genes and also occurred in the transgenic mouse, and these passenger genes may have functional consequences in various tissues.
该转基因小鼠广泛应用于脂肪细胞特异性遗传操作的发展，以研究肥胖和 2 型糖尿病。在利用脂肪细胞选择型转基因小鼠开发新的小鼠模型的过程中，发现了一个感兴趣的基因和转基因之间存在很强的遗传连锁。对转基因小鼠模型的全基因组测序确定了位于 9 号染色体上基因座内的基因组插入位点，该插入导致脂肪组织中基因表达显著下降。基因和也插入了转基因小鼠中，这些过客基因可能在各种组织中具有功能后果。
