Identification of the transgene insertion site for an adipocyte-specific adiponectin-cre model and characterization of the functional consequences.

The transgenic mouse is widely used in the development of adipocyte-specific genetic manipulations for the study of obesity and type 2 diabetes. In the process of developing a new mouse model utilizing the adipocyte selective transgenic mouse, strong genetic linkage between a gene of interest, , and the transgene was discovered. Whole-genome sequencing of the transgenic mouse model identified the genomic insertion site within the gene locus on chromosome 9 and this insertion causes a significant decrease in gene expression in adipose tissue. Insertion of genes and also occurred in the transgenic mouse, and these passenger genes may have functional consequences in various tissues.