Department of Oral and Maxillofacial Surgery, Dong-A University Medical Center, Busan, Korea.
Oral Surg Oral Med Oral Pathol Oral Radiol. 2012 May;113(5):583-92. doi: 10.1016/j.tripleo.2011.04.029. Epub 2011 Aug 10.
The objective of this histologic study was to evaluate platelet-rich fibrin (PRF)-mixed tricalcium phosphate (TCP) and recombinant human bone morphogenic protein 2 (rhBMP-2)-coated TCP in their potential to enhance bone regeneration in sinus elevation in rabbits as well as in their inflammatory features.
Bilateral round-shaped defects (diameter 8.0 mm) were formed in the maxillary anterior sinus walls of 36 New Zealand white rabbits. The defects were grafted with TCP only (control group), with rhBMP-2-coated TCP (experimental group A) and with PRF-mixed TCP (experimental group B). Each group included 12 rabbits. The animals were killed at 3 days, 1 week, 2 weeks, 4 weeks, 6 weeks, and 8 weeks. The specimens underwent decalcification and were stained for histologic analysis.
There were no significant differences in inflammatory features among the groups at 3 days or the first week after operation. In a histomorphometric analysis, the new bone formation ratio showed significant differentiation between groups A and B. The TCP-only control group showed a relatively lower bone formation ratio rather than the experimental groups. The PRF-mixed TCP group showed a larger bone formation area, compared with both the control group and group A.
In the results of the histologic evaluation (hematoxylin-eosin, Masson trichrome stain), the experimental groups A and B showed rapid bone formation, remodeling, and calcification in the second week. Moreover, there was a significant difference between those experimental groups and the control group in the new bone formation area at the fourth, sixth, and eighth weeks. The PRF-mixed TCP showed more rapid bone healing than the rhBMP-2-coated TCP or the TCP-only control.
本组织学研究的目的是评估富含血小板纤维蛋白（PRF）混合磷酸三钙（TCP）和重组人骨形态发生蛋白 2（rhBMP-2）涂层 TCP 在兔窦提升中增强骨再生的潜力及其炎症特征。
在 36 只新西兰白兔的上颌窦前壁形成双侧圆形缺损（直径 8.0mm）。仅用 TCP（对照组）、rhBMP-2 涂层 TCP（实验组 A）和 PRF 混合 TCP（实验组 B）移植这些缺损。每组包括 12 只兔子。动物在 3 天、1 周、2 周、4 周、6 周和 8 周时处死。标本经脱钙后行组织学分析。
在术后 3 天或第 1 周，各组之间的炎症特征无显著差异。在组织形态计量学分析中，A 组和 B 组的新骨形成率有显著差异。仅用 TCP 的对照组的骨形成率相对较低，而实验组则不然。PRF 混合 TCP 组的骨形成面积大于对照组和 A 组。
在组织学评价（苏木精-伊红、Masson 三色染色）的结果中，实验组 A 和 B 在第 2 周表现出快速的骨形成、重塑和钙化。此外，在第 4、6 和 8 周时，实验组与对照组在新骨形成面积方面有显著差异。PRF 混合 TCP 比 rhBMP-2 涂层 TCP 或仅用 TCP 的对照组显示出更快的骨愈合。
