Department of Applied Chemistry, Faculty of Food Science, Szent István University, Budapest, Hungary.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2021 Apr;38(4):670-680. doi: 10.1080/19440049.2020.1862424. Epub 2021 Feb 10.
In this study, fifty-four wheat flour and wheat-based products available on the Hungarian market were assessed for twelve mycotoxins. Prior to analysis, a multi-mycotoxin method using high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS) was developed and validated for wheat and wheat-based products. A simple extraction with acetonitrile/water/formic acid (79/20/1 v/v%) was used for sample preparation. The limits of quantitation (LOQ) were between 0.02 and 161 μg kg. Good linearity (r > 0.995) was achieved for all mycotoxins investigated. Recoveries varied between 88 and 120% at three concentration levels. Based on the low relative matrix effect (RSD < 0.15%) of the different wheat flour samples, matrix-matched calibration was used, which also proved its suitability in proficiency testing (z-scores: -0.6 for DON; +1.5 for OTA; -0.5 for ZEA). DON was the predominant mycotoxin, which contaminated 84% of the investigated samples. Metabolised forms of DON were found in spelt, durum flour and some wheat-based products (D3G in 4 samples, 15Ac-DON in 7 samples). T-2 and HT-2 were the second most frequently detected mycotoxins. All investigated samples complied with current European/Hungarian legislation.
本研究评估了匈牙利市场上的 54 种小麦粉和以小麦为基础的产品中的 12 种真菌毒素。在分析之前，开发并验证了一种用于小麦和以小麦为基础的产品的高效液相色谱-串联质谱（HPLC-MS/MS）多真菌毒素方法。简单的乙腈/水/甲酸（79/20/1 v/v%）提取用于样品制备。定量限（LOQ）在 0.02 至 161 μg kg 之间。所有研究的真菌毒素均具有良好的线性（r>0.995）。在三个浓度水平下，回收率在 88%至 120%之间变化。基于不同小麦粉样品的相对基质效应（RSD <0.15%）较低，使用基质匹配校准，这也证明了其在能力验证中的适用性（DON 的 z 分数为-0.6；OTA 的 z 分数为+1.5；ZEA 的 z 分数为-0.5）。DON 是主要的真菌毒素，污染了 84%的调查样品。在斯佩尔特小麦粉、硬粒小麦粉和一些以小麦为基础的产品中发现了 DON 的代谢产物（D3G 在 4 个样品中，15Ac-DON 在 7 个样品中）。T-2 和 HT-2 是第二常见的真菌毒素。所有调查的样品均符合现行的欧洲/匈牙利法规。
