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电生物刺激和银离子对猪成纤维细胞的影响。

Effects of electrical biostimulation and silver ions on porcine fibroblast cells.

机构信息

Department of Animal, Dairy and Veterinary Sciences, Utah State University, Logan, Utah, United States of America.

出版信息

PLoS One. 2021 Feb 10;16(2):e0246847. doi: 10.1371/journal.pone.0246847. eCollection 2021.

DOI:10.1371/journal.pone.0246847
PMID:33566869
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7875371/
Abstract

The medical applications of electrical biostimulation and silver ions have been evaluated in laboratory experiments and clinical studies for more than two decades. Their effects on preventing infection and promoting wound healing have been described. However, little is known about the role of electrical biostimulation and/or silver ion on changes in cellular transcriptome dynamics. To our knowledge, few studies have been conducted to investigate the potential of electrical biostimulation and silver ions in cell reprogramming. Besides, it is essential to assess any possible adverse effects or potential benefits of the silver ions on mammalian cells to address its safety concerns and to improve silver medical products. In this study, we investigated transcriptomic changes in porcine fibroblast cells in response to electrical biostimulation in the presence of silver ions. Exposed cells presented distinct morphological changes after treatment, which was mainly due to the exposure of silver ions rather than the electrical current itself. Gene expression analyses suggested that electrical biostimulation and silver ions did not increase the expression of pluripotency genes. Interestingly, a set of genes related to cellular metabolic processes were differentially expressed after cells were exposed to electrically generated silver ions for 21 hours. We found that 2.00 mg/L of electrically generated silver ion caused an increase of ATP generation and an increase of the total pool of NAD+ and NADH, while ROS production did not change. Aside from toxic effects, the results reported herein demonstrate the alternative effects of silver ions on mammalian cells, especially an oxidative phosphorylation burst. To our knowledge, this response of mammalian cells to silver ions has not been described previously. Although the function of this burst is not understood, it may lead to alterations in cellular activities such as metabolic remodeling and cell reprogramming, and/or serve an as-yet unknown function in neutralization or detoxification of the silver ions within the cells.

摘要

电生物刺激和银离子在医学中的应用已在实验室实验和临床研究中得到了二十多年的评估。它们在预防感染和促进伤口愈合方面的作用已被描述。然而,对于电生物刺激和/或银离子对细胞转录组动力学变化的影响知之甚少。据我们所知,很少有研究探讨电生物刺激和银离子在细胞重编程中的潜在作用。此外,评估银离子对哺乳动物细胞的任何潜在不良反应或潜在益处对于解决其安全性问题和改进银医疗产品至关重要。在这项研究中,我们研究了猪成纤维细胞在存在银离子的情况下对电生物刺激的转录组变化。处理后的细胞表现出明显的形态变化,这主要是由于银离子的暴露,而不是电流本身。基因表达分析表明,电生物刺激和银离子不会增加多能性基因的表达。有趣的是,一组与细胞代谢过程相关的基因在细胞暴露于电生成的银离子 21 小时后表达差异。我们发现,2.00mg/L 的电生成银离子会增加 ATP 的产生,并增加 NAD+和 NADH 的总池,而 ROS 的产生没有变化。除了毒性作用外,本文报告的结果还证明了银离子对哺乳动物细胞的替代作用,特别是氧化磷酸化爆发。据我们所知,哺乳动物细胞对银离子的这种反应以前尚未被描述过。虽然这种爆发的功能尚不清楚,但它可能导致细胞活动的改变,如代谢重塑和细胞重编程,以及/或在细胞内中和/或解毒银离子方面发挥未知的作用。

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