二维氢氧化钴纳米片作为氧化酶模拟物用于食品中亚硫酸盐的比色测定。
2D CoOOH nanosheets as oxidase mimic for the colorimetric assay of sulfite in food.
作者信息
Mei Tianxiao, Zhang Sheng, Sun Jie, Hu Yihui
机构信息
Institute for Regenerative Medicine, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China.
Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, China Pharmaceutical University, Nanjing 210009, China.
出版信息
Anal Methods. 2021 Feb 19;13(6):764-768. doi: 10.1039/d1ay00039j.
Here, we report a rapid, sensitive and selective colorimetric assay for sulfite (SO32-) based on the intrinsic oxidase-like activity of 2D cobalt oxyhydroxide nanosheets (CoOOH NSs). The 2D CoOOH nanozyme could directly oxidize 3,3',5,5'-tetramethylbenzidine (TMB) into blue products (TMBox) in an aerobic solution without H2O2. Interestingly, the presence of SO32- could effectively inhibit the CoOOH NS-O2-TMB reaction system and thus caused changes in color and absorbance, which facilitated a colorimetric sensor for sulfite. After optimizing detection conditions, a facile and robust approach was developed for SO32- detection in food.
在此，我们报道了一种基于二维氢氧化钴纳米片（CoOOH NSs）的固有类氧化酶活性的快速、灵敏且选择性的亚硫酸盐（SO32-）比色测定法。二维CoOOH纳米酶可在无氧溶液中直接将3,3',5,5'-四甲基联苯胺（TMB）氧化为蓝色产物（TMBox），无需过氧化氢。有趣的是，SO32-的存在可有效抑制CoOOH NS-O2-TMB反应体系，从而导致颜色和吸光度的变化，这有助于构建亚硫酸盐比色传感器。优化检测条件后，开发了一种简便且可靠的食品中亚硫酸盐检测方法。
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