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在人工自体皮肤模型中对临床常用防腐剂的细胞毒性和表皮屏障功能评估

Cytotoxicity and Epidermal Barrier Function Evaluation of Common Antiseptics for Clinical Use in an Artificial Autologous Skin Model.

作者信息

Quiñones-Vico María I, Fernández-González Ana, Pérez-Castejón Elena, Montero-Vílchez Trinidad, Arias-Santiago Salvador

机构信息

Cell Production and Tissue Engineering Unit, Virgen de las Nieves University Hospital, 18014 Granada, Spain.

Biosanitary Institute of Granada (ibs. GRANADA), 18014 Granada, Spain.

出版信息

J Clin Med. 2021 Feb 8;10(4):642. doi: 10.3390/jcm10040642.

DOI:10.3390/jcm10040642
PMID:33567522
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7914612/
Abstract

Bioengineered artificial skin substitutes (BASS) are the main treatment used in addition to autografts when skin injuries involve a large body surface area. Antiseptic/antibiotic treatment is necessary to prevent infections in the BASS implant area. This study aims to evaluate the effect of antiseptics and antibiotics on cell viability, structural integrity, and epidermal barrier function in BASS based on hyaluronic acid during a 28 day follow-up period. Keratinocytes (KTs) and dermal fibroblasts (DFs) were isolated from skin samples and used to establish BASS. The following antibiotic/antiseptic treatment was applied every 48 h: colistin (1%), chlorhexidine digluconate (1%), sodium chloride (0.02%), and polyhexanide (0.1%). Cell viability (LIVE/DEAD assay), structural integrity (histological evaluation), and epidermal barrier function (trans-epidermal water loss, (TEWL), Tewameter) were also evaluated. Cell viability percentage of BASS treated with chlorhexidine digluconate was significantly lower ( ≤ 0.001) than the other antiseptics at day 28. Compared to other treatments, chlorhexidine digluconate and polyhexanide significantly affected the epithelium. No significant differences were found regarding epidermal barrier. These results may be useful for treatment protocols after implantation of BASS in patients and evaluating them in clinical practice. BASS represent a suitable model to test in vitro the impact of different treatments of other skin wounds.

摘要

当皮肤损伤涉及大面积体表时，除自体移植外，生物工程人工皮肤替代物（BASS）是主要的治疗方法。在BASS植入区域进行抗菌/抗生素治疗对于预防感染是必要的。本研究旨在评估在28天的随访期内，防腐剂和抗生素对基于透明质酸的BASS中细胞活力、结构完整性和表皮屏障功能的影响。从皮肤样本中分离角质形成细胞（KTs）和真皮成纤维细胞（DFs），并用于构建BASS。每48小时进行以下抗生素/防腐剂处理：多粘菌素（1%）、葡萄糖酸氯己定（1%）、氯化钠（0.02%）和聚己双胍（0.1%）。还评估了细胞活力（活/死检测）、结构完整性（组织学评估）和表皮屏障功能（经表皮水分流失，TEWL，Tewameter）。在第28天，用葡萄糖酸氯己定处理的BASS的细胞活力百分比显著低于其他防腐剂（≤0.001）。与其他处理相比，葡萄糖酸氯己定和聚己双胍对上皮有显著影响。在表皮屏障方面未发现显著差异。这些结果可能有助于指导BASS植入患者后的治疗方案，并在临床实践中对其进行评估。BASS是一种合适的模型，可用于体外测试不同治疗方法对其他皮肤伤口的影响。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/957f/7914612/135d9b98dd47/jcm-10-00642-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/957f/7914612/f8b6c3dc27e8/jcm-10-00642-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/957f/7914612/b9be2dea9ef2/jcm-10-00642-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/957f/7914612/0265c36d6834/jcm-10-00642-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/957f/7914612/135d9b98dd47/jcm-10-00642-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/957f/7914612/f8b6c3dc27e8/jcm-10-00642-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/957f/7914612/61a4ecd05a80/jcm-10-00642-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/957f/7914612/4a3862a32c3a/jcm-10-00642-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/957f/7914612/05c93de71224/jcm-10-00642-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/957f/7914612/02479e39d717/jcm-10-00642-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/957f/7914612/09a361d66e81/jcm-10-00642-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/957f/7914612/b9be2dea9ef2/jcm-10-00642-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/957f/7914612/0265c36d6834/jcm-10-00642-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/957f/7914612/135d9b98dd47/jcm-10-00642-g009.jpg

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