Unit for Population-Based Dermatology Research, St John's Institute of Dermatology, Guy's and St Thomas' NHS Foundation Trust and King's College London, London, UK.
Skin Research Group, School of Medicine, Ninewells Hospital & Medical School, University of Dundee, Dundee, UK.
J Invest Dermatol. 2018 Nov;138(11):2295-2300.e1. doi: 10.1016/j.jid.2018.09.001.
Transepidermal water loss (TEWL) is the most widely used objective measurement for assessing the barrier function of skin in healthy individuals but also patients with skin diseases that are associated with skin barrier dysfunction, such as atopic dermatitis. TEWL is the quantity of condensed water that diffuses across a fixed area of stratum corneum to the skin surface per unit time. The water evaporating from the skin is measured using a probe that is placed in contact with the skin surface and contains sensors that detect changes in water vapor density. TEWL can be measured using an open-chamber, unventilated-chamber, or condenser-chamber device. It is a sensitive measure that is affected by properties of the surrounding microclimate such as environmental humidity, temperature, and airflow and should be measured under controlled conditions. TEWL varies significantly across different anatomical sites and also depends on sweat gland activity, skin temperature, and corneocyte properties. Here we describe how to optimally use TEWL measurements as a skin research tool in vivo and in vitro.
经皮水分丢失（TEWL）是评估健康个体皮肤屏障功能的最常用客观测量方法，但也可用于评估与皮肤屏障功能障碍相关的皮肤病患者，如特应性皮炎。TEWL 是单位时间内穿过角质层固定面积扩散到皮肤表面的冷凝水的量。通过将探针放置在与皮肤表面接触并包含检测水蒸气密度变化的传感器的探针来测量从皮肤蒸发的水分。TEWL 可以使用开放式腔室、非通风式腔室或冷凝器腔室设备进行测量。它是一种敏感的测量方法，受周围微气候的特性影响，如环境湿度、温度和气流，并且应该在受控条件下进行测量。TEWL 在不同的解剖部位差异很大，并且还取决于汗腺活动、皮肤温度和角质形成细胞特性。在这里，我们描述了如何将 TEWL 测量作为体内和体外皮肤研究工具进行优化使用。
