Potent immunosuppressive and anti-inflammatory bisindole alkaloids from .

Phytochemical investigation of led to a new aspidosperma-aspidosperma bisindole alkaloid (BIA), bis-19-hydroxyvenalstonidine (), together with three known BIAs (-). The structures were established by extensive analysis of their HRESIMS, NMR data, and comparing with the reported data. BIA is an almost symmetrical structure, linked by C3-C14' bond, while BIAs - are reported for the first time from the plant. The cytotoxic, immunosuppressive and anti-inflammatory activities of BIAs - were evaluated . BIAs , and showed good toxicity against MOLT-4 cell lines with IC values in the range of 1.5-17.5 M. BIA exhibited the strongest inhibitory effect against MCF-7 cell lines with an IC value of 7.1 μM. BIA significantly inhibited Con A-stimulated mice splenocytes proliferation equal to that of the positive control (DXM) in a concentration-dependent manner. BIAs and were able to decrease the NO production in LPS-induced RAW 264.7 cells at 30 μM concentration. BIA showed similar inhibition of nitric oxide release, compared to that of DXM. Furthermore, BIA remarkably inhibited the levels of IL-6 and TNF-α compared to the LPS induced group. Interestingly, BIA displayed an inhibitory effect on TNF-α production similar to that of dexamethasone at a concentration of 20 μM.