Vegetable and Fruit Improvement Center, Department of Horticultural Sciences, Texas A&M University, College Station, TX, 77843, USA.
Department of Electrical and Computer Engineering, Texas A&M University, College Station, TX, 77843, USA.
Plant Sci. 2021 Mar;304:110809. doi: 10.1016/j.plantsci.2020.110809. Epub 2020 Dec 25.
Melon (Cucumis melo L.) is an important diploid crop with a wide variety of flavors due to its distinct aromatic volatile organic compounds (VOC). To understand the development of VOC profiles during fruit development, we performed metabolomic and transcriptomic analysis of two cantaloupe varieties over the course of fruit development. A total of 130 metabolites were detected in fruit samples, and 449014207 reads were mapped to the melon genome. A total of 4469 differentially expressed genes in fruits were identified and used to visualize the transition of VOC and transcriptomic profiles during the fruit development. A shift of VOC profiles in both varieties was observed from early-fruit profiles enriched in C5-C8 lipid-derived VOCs to late-fruit profiles abundant in C9 lipid-derived VOCs, apocarotenoids, and esters. The shift coincided with the expression of specific isoforms of lipid and carotenoid metabolizing enzymes as well as transcription factors involved in fruit ripening, metabolite regulation, and hormone signaling.
甜瓜（Cucumis melo L.）是一种重要的二倍体作物，因其独特的芳香挥发性有机化合物（VOC）而具有多种风味。为了了解果实发育过程中 VOC 谱的发展，我们对两个哈密瓜品种在果实发育过程中的代谢组学和转录组学进行了分析。在果实样本中检测到 130 种代谢物，449014207 条reads 映射到甜瓜基因组。共鉴定出 4469 个在果实中差异表达的基因，并用于可视化 VOC 和转录组谱在果实发育过程中的变化。两种品种的 VOC 谱都发生了变化，从富含 C5-C8 脂质衍生 VOC 的早期果实谱转变为富含 C9 脂质衍生 VOC、类胡萝卜素和酯的晚期果实谱。这种转变与特定的脂质和类胡萝卜素代谢酶同工型以及参与果实成熟、代谢物调节和激素信号转导的转录因子的表达相吻合。
