Antibacterial efficacy, calcium ion release, and pH using calcium hydroxide with three vehicles.

CONTEXT

Vehicles combined with calcium hydroxide, as an intracanal medicament, play a key factor in affecting antibacterial, calcium release, and pH.

AIMS

The aim of this study is to investigate the effect of three vehicles (glycerin, chlorhexidine gluconate/CHX, and chitosan nanoparticle) combined with calcium hydroxide as an intracanal medicament on antibacterial efficacy against , calcium ion release, and pH of at different interval times of 7 and 14 days.

SETTINGS AND DESIGN

The research was experimental laboratory.

MATERIALS AND METHODS

Each study used 24 samples of eight teeth each and was randomly divided into three groups based on the vehicle of calcium hydroxide: group 1: glycerin, group 2: CHX, and group 3: chitosan nanoparticles. Each vehicle group was then further divided into two subgroups of four teeth based on the interval times (group A: 7 days and group B: 14 days). The antibacterial efficacy was determined using an agar diffusion method. Calcium ion release was analyzed with atomic absorption spectrometry, and pH was measured using a pH meter.

STATISTICAL ANALYSIS USED

Data from each study were analyzed by two-way ANOVA, followed by Tukey's test with a significance level of 95%.

RESULTS

The results exhibited that chitosan nanoparticles had the highest antibacterial efficacy against , calcium ion release, and pH, while the lowest was glycerin at 7 and 14 days ( < 0.05).

CONCLUSION

Calcium hydroxide combined with chitosan nanoparticle as an intracanal medicament produced the highest antibacterial efficacy against , calcium ion release, and pH than glycerin and CHX at intervals of 7 and 14 days.