Reddy N Bharath Naga, Sridhar Dinesh, Rajkumar Arasappan, Murugesan Sabari, Selvaraj Karthikeyan, Sankar Sathish
Department of Conservative Dentistry and Endodontics, Adhiparasakthi Dental College and Hospital, Melmaruvathur, Tamil Nadu Dr. MGR Medical University, Tamil Nadu, India, Phone: +91 9444792260, e-mail:
Department of Conservative Dentistry and Endodontics, Sri Venkateshwara Dental College, Pondicherry University, Puducherry, India.
J Contemp Dent Pract. 2020 Aug 1;21(8):905-909.
The aim of this study was to evaluate the antifungal efficacy of a novel endodontic irrigant octenidine against conventional irrigants sodium hypochlorite and EDTA on growth in the young and old population by calculating the number of colonies formed and by qualitative identification of dead/viable fungi by Confocal laser scanning microscopy (CLSM) method.
The total number of samples used in the study was eighty samples after decoronation of the crown portion the middle third of root canal. Each group was subdivided into four subgroups with various irrigation protocols: (A) 17% EDTA + 5.25% NaOCl, (B) 100% Octenisept, (C) 17% EDTA + 5.25% NaOCl + 1% clotrimazole, and (D) phosphate buffer saline. After completion of irrigation ATCC samples (90028) of were inoculated with 5 mL of peptone water each and incubated at 37°C for 72 hours to attain the turbidity corresponding to 0.5 McFarland standards CFU. Eight samples were analyzed for the formation of candidal colonies, and two samples for the assessment of viability of by confocal laser scanning microscope in each subgroup.
Comparison of antifungal efficacy of endodontic irrigants employed in the young and old populations revealed a significant reduction in the mean values of CFU and the mean values of percentage of non-viable microorganism by CLSM method. A positive relationship was revealed in the younger population which had a better antifungal efficacy than the older population in all the irrigant subgroups evaluated in the study.
All the endodontic irrigants employed in our study had a good antifungal efficacy against . Octenisept had a maximum antifungal efficacy, while phosphate saline showed the least efficacy in both age groups, which was quantitatively evaluated by CFU method, and the same was confirmed through qualitative evaluation by CLSM method.
plays a major role in the establishment and pathogenesis of failed root canal treatment. Age-related changes alter the adhesion potential of dentin, in turn influence the outcome of endodontic therapy. Octenidine, a novel antifungal agent, can be substituted over the conventionally used EDTA and NaOCl with less adverse effects.
本研究旨在通过计算菌落形成数量，并采用共聚焦激光扫描显微镜（CLSM）方法对死活真菌进行定性鉴定，评估新型牙髓冲洗剂奥替尼啶与传统冲洗剂次氯酸钠和乙二胺四乙酸（EDTA）相比，对年轻和老年人群真菌生长的抗真菌效果。
本研究中使用的样本总数为80个，均为根管中1/3冠部去除后的样本。每组再细分为四个亚组，采用不同的冲洗方案：（A）17% EDTA + 5.25%次氯酸钠，（B）100%奥替尼啶溶液，（C）17% EDTA + 5.25%次氯酸钠 + 1%克霉唑，（D）磷酸盐缓冲盐水。冲洗完成后，向每个样本接种5 mL蛋白胨水，并在37°C下孵育72小时，以达到对应于0.5麦氏标准CFU的浊度。每个亚组中，分析8个样本以观察念珠菌菌落的形成，2个样本用于通过共聚焦激光扫描显微镜评估其生存能力。
对年轻和老年人群使用的牙髓冲洗剂的抗真菌效果进行比较，结果显示通过CLSM方法，CFU平均值和非存活微生物百分比平均值均显著降低。在本研究评估的所有冲洗剂亚组中，年轻人群呈现出正相关关系，其抗真菌效果优于老年人群。
本研究中使用的所有牙髓冲洗剂对[具体真菌名称未给出]均具有良好的抗真菌效果。奥替尼啶溶液具有最大的抗真菌效果，而磷酸盐盐水在两个年龄组中显示出最小的效果，这通过CFU方法进行了定量评估，并通过CLSM方法进行定性评估得到了证实。
[具体真菌名称未给出]在根管治疗失败的发生和发病机制中起主要作用。与年龄相关的变化会改变牙本质的黏附潜力，进而影响牙髓治疗的结果。奥替尼啶作为一种新型抗真菌剂，可以替代传统使用的EDTA和次氯酸钠，且副作用较小。
