Department of Integrative Biology, Oregon State University, Corvallis, OR 97331, USA.
Department of Food Science and Technology, Oregon State University, Corvallis, OR 97331, USA.
Molecules. 2021 Apr 3;26(7):2064. doi: 10.3390/molecules26072064.
Tuna backbone peptide (TBP) has been reported to exert potent inhibitory activity against lipid peroxidation in vitro. Since this bears relevant physiological implications, this study was undertaken to assess the impact of peptide modifications on its bioactivity and other therapeutic potential using in vitro and in silico approach. Some TBP analogs, despite lower purity than the parent peptide, exerted promising antioxidant activities in vitro demonstrated by ABTS radical scavenging assay and cellular antioxidant activity assay. In silico digestion of the peptides resulted in the generation of antioxidant, angiotensin-converting enzyme (ACE), and dipeptidyl peptidase-IV (DPPIV) inhibitory dipeptides. Using bioinformatics platforms, we found five stable TBP analogs that hold therapeutic potential with their predicted multifunctionality, stability, non-toxicity, and low bitterness intensity. This work shows how screening and prospecting for bioactive peptides can be improved with the use of in vitro and in silico approaches.
金枪鱼脊柱骨肽 (TBP) 已被报道具有体外抑制脂质过氧化的强大活性。由于这具有相关的生理意义，本研究采用体外和计算方法评估肽修饰对其生物活性和其他治疗潜力的影响。一些 TBP 类似物，尽管纯度低于母体肽，但在 ABTS 自由基清除试验和细胞抗氧化活性试验中表现出有希望的抗氧化活性。肽的计算机消化导致产生抗氧化剂、血管紧张素转换酶 (ACE) 和二肽基肽酶-IV (DPPIV) 抑制二肽。使用生物信息学平台，我们发现了五个具有治疗潜力的稳定 TBP 类似物，它们具有预测的多功能性、稳定性、非毒性和低苦味强度。这项工作展示了如何通过使用体外和计算方法来改进生物活性肽的筛选和勘探。
