Lucius R, Büttner D W, Kirsten C, Diesfeld H J
Parasitology. 1986 Jun;92 ( Pt 3):569-80. doi: 10.1017/s0031182000065458.
Sera from 40 onchocerciasis patients from the Yemen Arab Republic with either mild localized forms of onchocerciasis, intermediate or severe localized forms of the disease or generalized forms of infection were studied with respect to their IgG and IgM response against Onchocerca volvulus antigens. Immunoblotting, performed with SDS-PAGE-separated proteins of female O. volvulus and quantified by densitometric scanning, revealed IgG and IgM antibodies against worm components in sera of all patients. Persons with intermediate or severe localized forms of onchocerciasis had a stronger IgG response against more proteins than individuals of the other groups. However, some antigens (Mr 21, 23, 30, 33 kDa) induced comparable quantities of IgG in all groups. The IgM response of patients with mild localized forms of onchocerciasis was more intensive and directed against more antigens than in the other groups. No antigens were detected that were recognized only by individuals with low levels of microfilaridermia. In all groups, varying concentrations of antibodies against cuticle, muscle/hypodermis layer and/or uterus of female O. volvulus were detected by the indirect immunofluorescence test using frozen worm sections as antigen. The highest mean antibody titres were found in patients with intermediate and severe localized forms of disease.
对来自阿拉伯也门共和国的40名盘尾丝虫病患者的血清进行了研究，这些患者患有轻度局限性盘尾丝虫病、中度或重度局限性盘尾丝虫病或全身性感染，研究内容为其针对旋盘尾丝虫抗原的IgG和IgM反应。采用十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳（SDS - PAGE）分离的雌性旋盘尾丝虫蛋白质进行免疫印迹，并通过光密度扫描定量，结果显示所有患者血清中均存在针对蠕虫成分的IgG和IgM抗体。患有中度或重度局限性盘尾丝虫病的患者比其他组的个体对更多蛋白质具有更强的IgG反应。然而，一些抗原（分子量21、23、30、33 kDa）在所有组中诱导产生的IgG量相当。轻度局限性盘尾丝虫病患者的IgM反应比其他组更强烈，且针对更多抗原。未检测到仅被微丝蚴血症水平低的个体识别的抗原。在所有组中，以冷冻虫体切片为抗原，通过间接免疫荧光试验检测到针对雌性旋盘尾丝虫角质层、肌肉/皮下层和/或子宫的不同浓度抗体。疾病中度和重度局限性形式的患者中平均抗体滴度最高。
