Shinoda J, Yamada H, Sakai N, Ando T, Hirata T, Miwa Y
Department of Neurosurgery, Gifu University School of Medicine, Japan.
J Neurosurg. 1988 May;68(5):710-20. doi: 10.3171/jns.1988.68.5.0710.
A sensitive enzyme-linked immunosorbent assay (ELISA) was used in a retrospective study of placental alkaline phosphatase (PLAP) levels in serum, cerebrospinal fluid (CSF), and intratumoral cyst fluid in primary intracranial germinoma. The ELISA showed no cross-reactivity with intestinal alkaline phosphatase except in very high concentrations, after samples had been heat-treated. Three patients with germinoma were studied for serum PLAP levels and in all the levels were elevated (3.78, 0.52, and 2.11 IU/liter). Two of the germinoma patients were studied for PLAP levels in the CSF, and both had elevated levels (0.83 and 9.83 IU/liter). The intratumoral cyst fluid in one case of germinoma was tested for PLAP and the level was found to be very high (603 IU/liter). These PLAP levels decreased concomitantly with the reduction in tumor size during irradiation. Serum PLAP levels were measured in 40 control adult male individuals and in the CSF of 20 nonpregnant patients with subarachnoid hemorrhage. The upper normal limits were 0.20 and 0.11 IU/liter in the serum and the CSF, respectively. All PLAP levels measured in the serum of patients with various brain tumors were 0.18 IU/liter or less. This study strongly suggests that PLAP is a clinically useful tumor marker for primary intracranial germinoma.
采用灵敏的酶联免疫吸附测定法（ELISA）对原发性颅内生殖细胞瘤患者血清、脑脊液（CSF）及肿瘤内囊液中的胎盘碱性磷酸酶（PLAP）水平进行回顾性研究。ELISA结果显示，除样本经过热处理且浓度非常高时外，该方法与肠碱性磷酸酶无交叉反应。对3例生殖细胞瘤患者的血清PLAP水平进行研究，结果显示所有患者的PLAP水平均升高（分别为3.78、0.52和2.11 IU/升）。对其中2例生殖细胞瘤患者的脑脊液PLAP水平进行研究，二者水平均升高（分别为0.83和9.83 IU/升）。对1例生殖细胞瘤患者的肿瘤内囊液进行PLAP检测，发现其水平非常高（603 IU/升）。在放疗期间，这些PLAP水平随肿瘤大小的缩小而相应降低。对40名成年男性对照个体的血清以及20例非妊娠蛛网膜下腔出血患者的脑脊液进行PLAP水平测定。血清和脑脊液中PLAP的正常上限分别为0.20和0.11 IU/升。对各类脑肿瘤患者血清中测得的所有PLAP水平均为0.18 IU/升或更低。本研究强烈提示，PLAP是原发性颅内生殖细胞瘤一种具有临床应用价值的肿瘤标志物。
