厄贝沙坦对自发性高血压大鼠的降压作用与改善瘦素-脂联素失衡有关。

The antihypertensive effect of irbesartan in spontaneously hypertensive rats is associated with improvement of the leptin-adiponectin imbalance.

作者信息

Weng Junting, Chen Min, Guo Rongjie, Yang Shuzhen, Liu Danjuan, Fang Dexiang

机构信息

Department of Critical Care Medicine, The Affiliated Hospital of Putian University , Putian, China.

出版信息

Adipocyte. 2021 Dec;10(1):101-107. doi: 10.1080/21623945.2021.1886409.

DOI:10.1080/21623945.2021.1886409

PMID:33570444

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7889210/

Abstract

OBJECTIVE

This study aimed to investigate whether the antihypertensive effect of irbesartan (IRB) in spontaneously hypertensive rats (SHR) was achieved through improvement of insulin resistance and adjustment of the LPN-APN imbalance.

METHODS

SHR rats were divided into SHAM, SHR-A and SHR-I group(8 per group). Homologous Wistar-Kyoto (WKY) rats were used as control group (WKY).The SHR-I group received 30 mg/kg/d IRB, the SHR-A group received 2.5 mg/kg AML. After 8 weeks, systolic blood pressure (SBP) was measured. The concentrations of blood glucose, insulin, LPN and APN were detected. Rat epididymal adipose tissues were collected to analyze the mRNA expression levels ofepididymal LPN and APN using reverse transcription-polymerase chain reaction. In addition, the LPN/APN ratio was calculated. Results:SBP, homeostasis model assessment of insulin resistance (HOMA-IR), LPN concentration, adipose LPN mRNA expression level, and the LPN/APN ratio increased (<0.05) and APN concentration and adipose APN mRNA expression level decreased (<0.05) in SHR rats.IRB decreased SBP, HOMA-IR, serum LPN, adipose LPN mRNA expression, and the LPN/APN ratio and increased serum APN and adipose APN mRNA expression.

CONCLUSION

The antihypertensive effect of IRB in SHR rats was associated with its improvement of insulin resistance and correction of the LPN-APN imbalance. ANOVA, one-way analysis of variance; SHR, Spontaneously hypertensive rats; WKY, Wistar kyoto rats; IRB, Irbesartan; AML, Amlodipine; LPN, Leptin; APN, Adiponectin; Ang-II, AngiotensinⅡ; HOMA-IR, Homoeostasis model assessment-insulin resistance; SBP, Systolic blood pressure; RT-PCR, Reverse transcription polymerase chain reaction; ARB, AngiotensinⅡreceptor blocker.

摘要

目的

本研究旨在探讨厄贝沙坦（IRB）对自发性高血压大鼠（SHR）的降压作用是否通过改善胰岛素抵抗和调节瘦素-脂联素失衡来实现。

方法

将SHR大鼠分为假手术组（SHAM）、SHR-A组和SHR-I组（每组8只）。选用同源的Wistar-Kyoto（WKY）大鼠作为对照组（WKY）。SHR-I组给予30 mg/kg/d的IRB，SHR-A组给予2.5 mg/kg的氨氯地平（AML）。8周后，测量收缩压（SBP）。检测血糖、胰岛素、瘦素（LPN）和脂联素（APN）的浓度。收集大鼠附睾脂肪组织，采用逆转录-聚合酶链反应分析附睾LPN和APN的mRNA表达水平。此外，计算LPN/APN比值。结果：SHR大鼠的SBP、胰岛素抵抗稳态模型评估（HOMA-IR）、LPN浓度、脂肪LPN mRNA表达水平和LPN/APN比值升高（<0.05），而APN浓度和脂肪APN mRNA表达水平降低（<0.05）。IRB降低了SBP、HOMA-IR、血清LPN、脂肪LPN mRNA表达和LPN/APN比值，并提高了血清APN和脂肪APN mRNA表达。

结论

IRB对SHR大鼠的降压作用与其改善胰岛素抵抗和纠正LPN-APN失衡有关。方差分析（ANOVA），单因素方差分析；SHR，自发性高血压大鼠；WKY，Wistar Kyoto大鼠；IRB，厄贝沙坦；AML，氨氯地平；LPN，瘦素；APN，脂联素；Ang-II，血管紧张素Ⅱ；HOMA-IR，胰岛素抵抗稳态模型评估；SBP，收缩压；RT-PCR，逆转录聚合酶链反应；ARB，血管紧张素Ⅱ受体阻滞剂。