Post-Graduate Program in Pharmaceutical Sciences, State University of Maringá, Maringá, Brazil.
Department of Chemistry, State University of Maringá, Maringá, Brazil.
Metab Syndr Relat Disord. 2021 Jun;19(5):296-304. doi: 10.1089/met.2020.0116. Epub 2021 Feb 10.
We previously established that male Swiss mice () receiving a high-fat diet HFD) during 8 weeks exhibit similar caloric ingestion and body weight (grams) compared with mice fed a high-carbohydrate diet (HCD). HFD mice exhibit a lower inflammatory state than an HCD in the liver, skeletal muscle, and brain. In addition, we demonstrated that HFD and HCD modulated fatty acids (FA) composition in these tissues. In this study, our objective was to compare HFD mice and HCD mice in terms of systemic inflammation. Saturated FA (SFA), monounsaturated FA, omega-6 polyunsaturated FA (n-6 PUFA), and n-3 PUFA were evaluated at the time points 0, 1, 7, 14, 28, and 56 days after starting the administration of the diets. We investigated n-6 PUFA:n-3 PUFA, SFA:n-3 PUFA, palmitic acid:α-linolenic acid (ALA), and myristic acid:docosahexaenoic acid (DHA) ratios as potential serum biomarkers of systemic inflammation. We also measured the serum levels of basic fibroblast growth factor, granulocyte-macrophage colony-stimulating factor (GM-CSF), inducible protein 10 (IP-10), interferon gamma (IFN-γ), interleukin (IL)-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-13, IL-17, macrophage inflammatory protein-1α (MIP-1-α), monocyte chemotactic protein 1 (MCP-1), monokine induced by IFN-γ (MIG), and tumor necrosis factor α (TNF-α). The HFD group had lower ( < 0.05) n-6 PUFA:n-3 PUFA, palmitic acid:ALA, myristic acid:DHA ratios, and lower plasma levels of proinflammatory cytokines (IFN-γ, MIG, GM-CSF, and IL-6). The HFD mice showed lower systemic inflammation compared with a caloric ingestion-body weight-matched control HCD mice.
我们之前已经证实，接受 8 周高脂肪饮食（HFD）的雄性瑞士小鼠与高碳水化合物饮食（HCD）喂养的小鼠相比，热量摄入和体重（克）相似。与 HCD 相比，HFD 小鼠的肝脏、骨骼肌和大脑中的炎症状态较低。此外，我们还证明 HFD 和 HCD 调节了这些组织中的脂肪酸（FA）组成。在这项研究中，我们的目的是比较 HFD 小鼠和 HCD 小鼠在全身炎症方面的差异。在开始饮食治疗后的 0、1、7、14、28 和 56 天，评估了饱和脂肪酸（SFA）、单不饱和脂肪酸、ω-6 多不饱和脂肪酸（n-6 PUFA）和 n-3 PUFA。我们研究了 n-6 PUFA:n-3 PUFA、SFA:n-3 PUFA、棕榈酸：α-亚麻酸（ALA）和肉豆蔻酸：二十二碳六烯酸（DHA）的比值作为全身炎症的潜在血清生物标志物。我们还测量了血清中碱性成纤维细胞生长因子、粒细胞-巨噬细胞集落刺激因子（GM-CSF）、诱导蛋白 10（IP-10）、干扰素γ（IFN-γ）、白细胞介素（IL）-1α、IL-1β、IL-2、IL-4、IL-5、IL-6、IL-10、IL-13、IL-17、巨噬细胞炎症蛋白-1α（MIP-1-α）、单核细胞趋化蛋白 1（MCP-1）、IFN-γ诱导的单核细胞趋化蛋白（MIG）和肿瘤坏死因子α（TNF-α）的水平。与 HCD 组相比，HFD 组的 n-6 PUFA:n-3 PUFA、棕榈酸：ALA、肉豆蔻酸：DHA 比值较低（<0.05），促炎细胞因子（IFN-γ、MIG、GM-CSF 和 IL-6）的血浆水平也较低。与热量摄入-体重匹配的 HCD 对照组相比，HFD 小鼠的全身炎症水平较低。
