Unidad de Investigación y Desarrollo en Alimentos (UNIDA), Tecnológico Nacional de México/Instituto Tecnológico de Veracruz, Veracruz, Mexico.
Laboratorio de Química y Biotecnología de Productos Lácteos, Centro de Investigación en Alimentación y Desarrollo A. C., Hermosillo, Mexico.
J Sci Food Agric. 2021 Sep;101(12):5049-5055. doi: 10.1002/jsfa.11149. Epub 2021 Feb 27.
Previous studies have demonstrated the acrylamide-removing properties of probiotic monocultures; however, potential advantages of consortia over monocultures in reducing the dietary exposure to acrylamide have not been proven. Hence this work aims to assess the acrylamide (AA)-binding properties of bacterial consortia, consisting of either probiotic strains and / or representative bacteria of duodenal microbiota, exposed to simulated gastrointestinal conditions (SGC). The AA binding capacity of ten probiotic strains (PS) and six duodenal strains (NDS) was evaluated under different conditions; then, three different consortia (PS, NDS, and PS + NDS) were assessed under SGC.
Among individual PS, Bacillus coagulans GBI-30, Lactobacillus fermentum J23, L. pentosus J37 and J24, and L. casei Shirota, exhibited the highest AA-binding capacity (80-87%), while Bifidobacterium catenulatun ATCC27676, Streptococcus salivarius subsp. thermophilus ATCC19258, and S. gallolyticus ATCC9809 were the best (ca. 68%) NDS monocultures. Probiotic strain consortia showed higher (P < 0.05) AA binding capacity (> 90%) than monoculture bacteria. Conversely, individual NDS cultures displayed higher (P < 0.05) binding capacity than NDS consortia (60%). A significant reduction (P < 0.05) in AA removal capacity was observed when consortia were exposed to SGC, PS consortia being the most effective (> 60% removal).
These results suggest that consortia of specific PS could play an important role in reducing the intestinal availability of acrylamide. © 2021 Society of Chemical Industry.
先前的研究已经证明了益生菌的单培养物具有去除丙烯酰胺的特性；然而，与单培养物相比， consortium 在降低膳食中丙烯酰胺暴露方面的潜在优势尚未得到证实。因此，本研究旨在评估暴露于模拟胃肠道条件（SGC）下的益生菌和/或十二指肠微生物群代表细菌组成的细菌 consortium 的丙烯酰胺（AA）结合特性。在不同条件下评估了 10 株益生菌（PS）和 6 株十二指肠菌（NDS）的 AA 结合能力；然后，在 SGC 下评估了三种不同的 consortium（PS、NDS 和 PS+NDS）。
在单独的 PS 中，凝结芽孢杆菌 GBI-30、发酵乳杆菌 J23、J37 和 J24 以及干酪乳杆菌 Shirota 表现出最高的 AA 结合能力（80-87%），而双歧杆菌 catenulatun ATCC27676、唾液链球菌乳亚种。嗜热亚种 ATCC19258 和 S. gallolyticus ATCC9809 是最好的（约 68%）NDS 单培养物。益生菌菌株 consortium 显示出更高（P<0.05）的 AA 结合能力（>90%）高于单培养细菌。相反，个体 NDS 培养物显示出更高（P<0.05）的结合能力高于 NDS consortium（60%）。当 consortium 暴露于 SGC 时，AA 去除能力显著降低（P<0.05），PS consortium 的效果最为显著（>60%去除）。
这些结果表明，特定 PS 的 consortium 可能在降低肠道中丙烯酰胺的可用性方面发挥重要作用。©2021 化学工业协会。
