Department of Soil, Plant and Food Sciences, University of Bari Aldo Moro, 70126 Bari, Italy.
Faculty of Science and Technology, Free University of Bozen, 39100 Bolzano, Italy.
Nutrients. 2021 Mar 19;13(3):992. doi: 10.3390/nu13030992.
This work aimed to define the microbial consortia that are able to digest gluten into non-toxic and non-immunogenic peptides in the human gastrointestinal tract.
131 out of 504 tested and lactic acid bacteria, specifically (64), lactobacilli (63), (1), and (3), showed strong gastrointestinal resistance and were selected for their PepN, PepI, PepX, PepO, and PepP activities toward synthetic substrates. Based on multivariate analysis, 24 strains were clearly distinct from the other tested strains based on having the highest enzymatic activities. As estimated by RP-HPLC and nano-ESI-MS/MS, 6 cytoplasmic extracts out of 24 selected strains showed the ability to hydrolyze immunogenic epitopes, specifically 57-68 of α9-gliadin, 62-75 of A-gliadin, 134-153 of γ-gliadin, and 57-89 (33-mer) of α2-gliadin. Live and lysed cells of selected strains were combined into different microbial consortia for hydrolyzing gluten under gastrointestinal conditions. Commercial proteolytic enzymes ( E1, E2, Veron HPP, and Veron PS proteases) were also added to each microbial consortium. Consortium activity was evaluated by ELISA tests, RP-HPLC-nano-ESI-MS/MS, and duodenal explants from celiac disease patients.
two microbial consortia (Consortium 4: (.) DSM33363 and DSM33364, (.) DSM33373, DSM33298, and DSM33301; and Consortium 16: . DSM33363 and DSM33364, . DSM33373, DSM33374, DSM33300, DSM33297 and DSM33355), containing commercial enzymes, were able to hydrolyze gluten to non-toxic and non-immunogenic peptides under gastrointestinal conditions.
the results of this study provide evidence that selected microbial consortia could potentially improve the digestion of gluten in gluten-sensitive patients by hydrolyzing the immunogenic peptides during gastrointestinal digestion.
定义能够在人体胃肠道中将面筋消化成无毒和非免疫原性肽的微生物群落。
在 504 种测试的乳酸菌和乳酸杆菌中，有 131 种（64 种）、乳杆菌（63 种）、（1 种）和（3 种）具有较强的胃肠道抵抗力，并因其对合成底物的 PepN、PepI、PepX、PepO 和 PepP 活性而被选择。基于多元分析，根据最高酶活性，24 株与其他测试菌株明显不同。根据 RP-HPLC 和 nano-ESI-MS/MS 估计，24 株选定菌株中有 6 株胞质提取物具有水解免疫原性表位的能力，特别是α9-麦胶蛋白的 57-68、A-麦胶蛋白的 62-75、γ-麦胶蛋白的 134-153 和α2-麦胶蛋白的 57-89（33 肽）。选择的活细胞和裂解细胞被组合成不同的微生物群落，以在胃肠道条件下水解面筋。还向每个微生物群落中添加了商业蛋白水解酶（E1、E2、Veron HPP 和 Veron PS 蛋白酶）。通过 ELISA 试验、RP-HPLC-nano-ESI-MS/MS 和乳糜泻患者的十二指肠外植体评估群落活性。
两个微生物群落（群落 4：（。）DSM33363 和 DSM33364、（。）DSM33373、DSM333298 和 DSM33301；和群落 16：（。）DSM33363 和 DSM33364、（。）DSM33373、DSM33374、DSM33300、DSM33297 和 DSM33355），含有商业酶，能够在胃肠道条件下将面筋水解成无毒和非免疫原性肽。
本研究结果提供了证据表明，选定的微生物群落通过在胃肠道消化过程中水解免疫原性肽，有可能改善面筋敏感患者对面筋的消化。
