School of Medicine, College of Medicine, Fu Jen Catholic University, New Taipei 242062, Taiwan.
Department of Applied Chemistry, Chaoyang University of Technology, Taichung 413310, Taiwan.
Biomolecules. 2021 Jan 29;11(2):187. doi: 10.3390/biom11020187.
Kaempferitrin is extracted in significantly high quantities from the leaves of , which belongs to a group of plant species that comes under the genus Cinnamomum, well-known for its established anti-diabetic property in Chinese medicine. Oral administration of kaempferitrin and extract reduced blood sugar in alloxan-induced diabetic rats and improved the lipid profile in hamsters respectively. In this paper we studied the differential protein expression profile using mass spectrometry approach in the kaempferitrin-treated conditioned medium of liver cancer cell line HepG2. We discovered that 33 genes were up/down-regulated consistently between two biological samples. A slightly different version of the analysis software selected 28 genes, and the final 18 genes that appeared in both lists were selected. Interestingly, 5 proteins out of 18 were either exosomal markers or reported in high frequency of occurrence in exosome/secreted vesicles. We also examined the extracellular particles with atomic force microscopy (AFM), which showed that the conditioned medium of kaempferitrin treated had larger vesicles and fewer small vesicles. Expression of some lipid-regulating genes were also altered. Our data suggested that extracellular vesicle secretions may be regulated by kaempferitrin, and regulation of lipid profile by kampeferitrin involves multiple mechanisms.
山柰酚大量存在于山柰属植物的叶子中，该属植物是肉桂属植物的一种，因其在中医药中具有已确立的抗糖尿病特性而闻名。山柰酚和提取物的口服给药可降低四氧嘧啶诱导的糖尿病大鼠的血糖，并分别改善仓鼠的脂质谱。在本文中，我们使用质谱方法研究了山柰酚处理肝癌细胞系 HepG2 的条件培养基中的差异蛋白质表达谱。我们发现，两个生物样本之间有 33 个基因被一致地上调/下调。略有不同版本的分析软件选择了 28 个基因，最终出现在两个列表中的 18 个基因被选中。有趣的是，18 个基因中的 5 个蛋白要么是外泌体标记物，要么在外泌体/分泌小泡中高频出现。我们还使用原子力显微镜 (AFM) 检查了细胞外颗粒，结果表明，经山柰酚处理的条件培养基中有更大的囊泡和更少的小囊泡。一些脂质调节基因的表达也发生了改变。我们的数据表明，细胞外囊泡的分泌可能受到山柰酚的调节，而山柰酚对脂质谱的调节涉及多种机制。
