Shoji Toshihiko, Obara Mina, Takahashi Tadashi, Masumoto Saeko, Hirota Hironaka, Miura Tomisato
Food Research Institute, National Agriculture and Food Research Organization, 2-1-12, Kannondai, Tsukuba, Ibaraki 305-8605, Japan.
Hirosaki Industrial Research Institute, Aomori Prefectural Industrial Technology Research Center, 1-1-8, Ougi-machi, Hirosaki-shi, Aomori 036-8104, Japan.
Foods. 2021 Jan 29;10(2):274. doi: 10.3390/foods10020274.
Previously, we reported that apple polyphenols and their major active compounds, the flavan-3-ols and the procyanidins, can result in various health benefits in animals and humans, according to clinical studies. Here, we developed a rapid method for quantifying flavan-3-ols and procyanidins using high-performance liquid chromatography with fluorescence detection, where we investigated the amounts of flavan-3-ols and procyanidins in the Japanese major apple production centre, the Aomori Prefecture, from 2016 to 2018. The non-bagged 'Fuji ( = 609)', the bagged 'Fuji ( = 1101)', and the 'Orin ( = 504)' apples were evaluated in terms of their differences in flavan-3-ols and procyanidins based on apple variety and the controlled atmosphere storage. The bagging treatments of the 'Fuji' apples resulted in significantly higher concentrations of procyanidins, while changes in flavan-3-ols concentrations were not clearly observed by treatment. In addition, 'Orin' had a significantly higher concentration of procyanidins than that of 'Fuji'. In contrast, the controlled atmosphere storage hardly caused any changes in the flavan-3-ol and procyanidin contents. Hence, we present the concentrations of flavan-3-ols and procyanidins in major Japanese apples using the rapid high-performance liquid chromatography method with fluorescence detection.
此前，据临床研究报道，我们发现苹果多酚及其主要活性成分黄烷 - 3 - 醇和原花青素对动物和人类具有多种健康益处。在此，我们开发了一种使用高效液相色谱 - 荧光检测法定量黄烷 - 3 - 醇和原花青素的快速方法，并于2016年至2018年调查了日本主要苹果产区青森县的黄烷 - 3 - 醇和原花青素含量。对未套袋的“富士（= 609）”、套袋的“富士（= 1101）”和“王林（= 504）”苹果，根据苹果品种和控制气氛贮藏条件，评估了它们在黄烷 - 3 - 醇和原花青素含量上的差异。“富士”苹果的套袋处理使原花青素浓度显著升高，而处理后黄烷 - 3 - 醇浓度变化不明显。此外，“王林”的原花青素浓度显著高于“富士”。相比之下，控制气氛贮藏几乎未引起黄烷 - 3 - 醇和原花青素含量的任何变化。因此，我们使用高效液相色谱 - 荧光检测快速方法给出了日本主要苹果中黄烷 - 3 - 醇和原花青素的含量。
