Department of Food Technology, University of Campinas, Campinas, Brazil.
Food Science Department, University of Campinas, Campinas, Brazil.
Food Sci Technol Int. 2022 Jan;28(1):85-92. doi: 10.1177/1082013221992665. Epub 2021 Feb 11.
The aims of this study were to evaluate the occurrence of lostridium in commercial raw meat and meat products commercialized in Brazil, and to determine the pathogenic potential and antimicrobial susceptibility of the isolates. After selective enrichment, the isolation of involved plating with and without an alcohol shock treatment onto moxalactam agar (CDMNA). The toxigenic profile was determined through PCR for detection of and genes and an enzyme-linked immunosorbent assay for toxin A/B. was isolated from 8.9% (17 out of 192) of analyzed samples. Plating without alcohol treatment (sensitivity of 88.23%) was more efficient than with alcohol treatment (sensitivity of 29.41%) in isolation. The profile A + B+CDT- was observed in 35.0% (28/80) of the isolates. The A/B toxin was tested in 44 isolates and 15.9% of them were positive. Resistance to clindamycin, ceftizoxime tetracycline, metronidazole, vancomycin, and ceftriaxone were observed among isolates. Multi-drug resistance was detected in 36.4% (8/22) of the isolates evaluated.
本研究的目的是评估商业生肉和肉类产品中梭菌的发生情况，并确定分离株的致病潜力和抗菌药物敏感性。经过选择性富集后，通过在莫西沙酮琼脂（CDMNA）上进行有和没有酒精冲击处理的平板划线来进行分离。通过 PCR 检测 、 基因和酶联免疫吸附试验（ELISA）检测毒素 A/B 来确定产毒谱。从 192 个分析样本中的 8.9%（17 个）中分离出了梭菌。不进行酒精处理的平板划线（敏感性为 88.23%）比进行酒精处理的平板划线（敏感性为 29.41%）更有效地分离梭菌。在 80 个分离株中的 35.0%（28/80）观察到 A+B+CDT- 谱。对 44 个分离株进行了 A/B 毒素检测，其中 15.9%为阳性。在分离株中观察到对克林霉素、头孢唑肟、四环素、甲硝唑和头孢曲松的耐药性。在所评估的 22 个分离株中的 36.4%（8/22）中检测到多药耐药性。
