Department of Ecology, College of Resources and Environmental Sciences, China Agricultural University, Beijing, P. R. China.
Plant Signal Behav. 2021 Apr 3;16(4):1886490. doi: 10.1080/15592324.2021.1886490. Epub 2021 Feb 12.
Mobile wound signals transmitted from local damaged to distal undamaged sites induce upsurge of jasmonic acid (JA) and activation of core JA signaling, priming the whole plant for broad-spectrum resistance/immunity against future challenges. We recently characterized two jasmonate importers AtJAT3 and AtJAT4 in jasmonate transporter (JAT) family that cooperatively regulate the transmission of JA from leaf-to-leaf in this wound-induced systemic response/resistance (WSR). As half-molecule ATP-binding cassette transporters, AtJAT3 and AtJAT4 need to form homodimers or/and heterodimer to function. Here we show interactions in AtJAT3-AtJAT3, AtJAT3-AtJAT4, and AtJAT4-AtJAT4 pairs by both yeast two-hybrid and bimolecular fluorescent complementation assays. Furthermore, we propose a model in which the homo-/hetero-dimers of AtJAT3/AtJAT4 mediated cell-cell transport of JA drives long-distance transmission of JA signal in a self-propagation mode and give perspectives on future works to reinforce this model.
从局部损伤部位向远端未损伤部位传输的移动性伤口信号诱导茉莉酸（JA）的激增和核心 JA 信号的激活，使整个植物对未来的挑战产生广谱抗性/免疫。我们最近在茉莉酸转运体（JAT）家族中鉴定了两个茉莉酸载体蛋白 AtJAT3 和 AtJAT4，它们共同调节这种诱导性系统性反应/抗性（WSR）中 JA 从叶片到叶片的传递。作为半分子 ATP 结合盒转运蛋白，AtJAT3 和 AtJAT4 需要形成同源二聚体或/和异源二聚体才能发挥作用。在这里，我们通过酵母双杂交和双分子荧光互补测定显示了 AtJAT3-AtJAT3、AtJAT3-AtJAT4 和 AtJAT4-AtJAT4 对之间的相互作用。此外，我们提出了一个模型，其中 AtJAT3/AtJAT4 的同源/异源二聚体介导 JA 的细胞-细胞运输，以自我传播模式驱动 JA 信号的长距离传递，并为未来的工作提供了加强该模型的视角。
