反复的机械损伤通过茉莉酸增强了抗性。

Repeated mechanical damage enhanced resistance to through jasmonic acid.

作者信息

Chen Yingying, Liang Shenghua, Wang Shuyao, Li Baocai, Wang Kun, Zhu Yongjin, Yang Risheng, Hao Xin, Yang Zhuoying, Shen Yingbai, Jiang Rihong, Li Kaixiang

机构信息

Guangxi Key Laboratory of Special Non-wood Forests Cultivation and Utilization, Guangxi Xylophyta Spices Research Center of Engineering Technology, Illicium and Cinnamomum Engineering Technology Research Center of National Forestry and Grassland Administration, Guangxi Forestry Research Institute, Nanning, China.

College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China.

出版信息

Front Plant Sci. 2023 Aug 8;14:1183002. doi: 10.3389/fpls.2023.1183002. eCollection 2023.

DOI:10.3389/fpls.2023.1183002

PMID:37615021

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10442551/

Abstract

INTRODUCTION

The leaf-chewing pest severely threatens the growth and development of . In our previous study, we found that mechanical damage (MD) to stem enhanced sapling resistance to larvae.

METHODS

To reveal the defense mechanisms underlying this observation, we analyzed the types and contents of volatile organic compounds (VOCs), phytohormone contents, and expression of phytohormone-related genes in response to MD and herbivory wounding(HW).

RESULTS

Here, we identified several VOCs, such as the pesticides fenobucarb and 2,4-di-tert-butylphenol, in mature leaf (ML) of MD-treated plants. Compared with salicylic acid (SA) or the ethylene (ET) pathway, jasmonic acid (JA) content and JA-related genes were more strongly upregulated. Interestingly, we found a dramatic difference between JA-related upstream and downstream genes expression in YL and ML, which confirmed that JA-Ile accumulation in MD-ML and HW-ML could be derived from local damaged site.

DISCUSSION

Taken together, we provide evidence that the JA pathway plays a dominant role in the response to MD and HW.

摘要

引言

食叶害虫严重威胁着[植物名称]的生长发育。在我们之前的研究中，我们发现对茎干的机械损伤（MD）增强了树苗对[害虫名称]幼虫的抗性。

方法

为揭示这一现象背后的防御机制，我们分析了挥发性有机化合物（VOCs）的类型和含量、植物激素含量以及响应MD和食草动物啃食伤口（HW）的植物激素相关基因的表达。

结果

在此，我们在MD处理植物的成熟叶片（ML）中鉴定出了几种VOCs，如农药仲丁威和2,4-二叔丁基苯酚。与水杨酸（SA）或乙烯（ET）途径相比，茉莉酸（JA）含量和JA相关基因的上调更为强烈。有趣的是，我们发现幼叶（YL）和成熟叶（ML）中JA相关的上游和下游基因表达存在显著差异，这证实了MD-ML和HW-ML中JA-Ile的积累可能源自局部受损部位。