Key Laboratory for Organic Electronics and Information Displays (KLOEID) & Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials (IAM), Jiangsu National Synergistic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts and Telecommunications, Nanjing, Jiangsu 210023, China.
Vascular Surgery Division, Karamay Central Hospital of Xinjiang, Karamay, Xinjiang 834000, China.
Biomacromolecules. 2021 Mar 8;22(3):1137-1146. doi: 10.1021/acs.biomac.0c01567. Epub 2021 Feb 12.
Integrated theranostic nanoplatforms with biomarker recognition and photothermal- and photodynamic (PTT/PDT) therapy is in high demand but remains challenging. Herein, a "sense-and-treat" nanoplatform based on semiconducting polymer nanoparticles (SPNs) for ratiometric bioimaging of phospholipase D (PLD) activity and PTT/PDT combined therapy was proposed. Semiconducting polymer nanoparticles (PSBTBT NPs) serve not only as photothermal agents but also as fluorescent quenchers of Rhodamine B (Rhod B) through a PLD-cleavable linker. Chlorin e6 (Ce6) was used as a photodynamic agent and fluorescence reference. The obtained nanoplatform (PSBTBT-Ce6@Rhod NPs) showed high PDT efficiency and photothermal performance upon single laser irradiation. The PTT/PDT combined therapy achieved more efficient tumor inhibition results as compared with single treatments. In addition, the overexpressed biomarker PLD in tumor tissue will cleave Rhod, leading to the fluorescence recovery of Rhod B and thus allowing the activatable fluorescence imaging of tumor and targeted phototherapy.
用于磷脂酶 D (PLD) 活性比率生物成像和光热-光动力 (PTT/PDT) 联合治疗的基于半导体聚合物纳米粒子 (SPNs) 的“感知-治疗”纳米平台具有很高的需求，但仍然具有挑战性。在此，提出了一种基于半导体聚合物纳米粒子 (PSBTBT NPs) 的“感知-治疗”纳米平台，用于磷脂酶 D (PLD) 活性的比率生物成像和光热-光动力 (PTT/PDT) 联合治疗。半导体聚合物纳米粒子 (PSBTBT NPs) 不仅可用作光热剂，还可用作通过 PLD 可切割连接体猝灭罗丹明 B (Rhod B) 的荧光剂。氯 e6 (Ce6) 被用作光动力剂和荧光参比。所得纳米平台 (PSBTBT-Ce6@Rhod NPs) 在单激光照射下表现出高 PDT 效率和光热性能。与单一治疗相比，PTT/PDT 联合治疗可实现更有效的肿瘤抑制效果。此外，肿瘤组织中过表达的生物标志物 PLD 将切割 Rhod，导致 Rhod B 的荧光恢复，从而实现肿瘤的激活荧光成像和靶向光疗。
