Projan S J, Moghazeh S, Novick R P
Department of Plasmid Biology, Public Health Research Institute, New York, NY 10016.
Nucleic Acids Res. 1988 Mar 25;16(5):2179-87. doi: 10.1093/nar/16.5.2179.
pS194 is a naturally occurring Staphylococcus aureus plasmid encoding streptomycin resistance. The plasmid has a copy number of about 25 per cell, and belongs to the inc5 incompatibility group. The nucleotide sequence of pS194 has been determined and consists of 4397 base pairs including four open reading frames potentially encoding proteins of greater than 100 amino acids. All four of these reading frames are on the same coding strand. The first reading frame, repE, encodes a 38 kd protein specifically required for pS194 replication. The second open reading frame, str, encodes a 34 kd polypeptide required for streptomycin resistance, probably a streptomycin adenylyltransferase. The third potential polypeptide, rlx, would be 37 kd and is probably required for relaxation complex formation and plasmid mobilization by conjugative plasmids. The fourth, orfD, overlapping the rlx reading frame, is potentially 27 kd, and may also be involved in mobilization.
pS194是一种天然存在的金黄色葡萄球菌质粒，编码链霉素抗性。该质粒每个细胞的拷贝数约为25，属于Inc5不相容群。pS194的核苷酸序列已被确定，由4397个碱基对组成，包括四个开放阅读框，可能编码超过100个氨基酸的蛋白质。所有这四个阅读框都在同一编码链上。第一个阅读框repE编码一个38kd的蛋白质，是pS194复制所特需的。第二个开放阅读框str编码一个34kd的多肽，是链霉素抗性所必需的，可能是一种链霉素腺苷酸转移酶。第三个潜在的多肽rlx为37kd，可能是形成松弛复合体和通过接合性质粒进行质粒转移所必需的。第四个orfD与rlx阅读框重叠，可能为27kd，也可能参与转移过程。
