Department of Physiology, ''Iuliu Hatieganu'' University of Medicine and Pharmacy, 1-3 Clinicilor Street, 400006, Cluj-Napoca, Romania.
Department of Physiology, ''Iuliu Hatieganu'' University of Medicine and Pharmacy, 1-3 Clinicilor Street, 400006, Cluj-Napoca, Romania.
Food Chem Toxicol. 2021 Apr;150:112053. doi: 10.1016/j.fct.2021.112053. Epub 2021 Feb 9.
To investigate the effects of prenatal exposure to AgNPs obtained by green synthesis with Viburnum opulus L. extract on the testis in male offspring rats.
Two different doses of AgNPs (0.8 and 1.5 mg/kg b.w.) and vehicle (PBS) were administered to Wistar female rats on days 3-14 of gestation. At 6 weeks after birth, the ultrastructural changes in correlation with the amount of silver as well as the parameters of oxidative stress, inflammation and cell death mechanisms in the testis of male offspring were evaluated.
AgNPs administered during pregnancy crossed the placental and testicular barriers and induced oxidative stress, DNA damage and autophagy as mechanism of cell toxicity. The markers of inflammation and apoptosis decreased after AgNPs exposure while the NFkB activation increased. TEM examination revealed important ultrastructural changes of Sertoli cells, numerous vacuoles and cytoplasmic changes suggestive of the cell's evolution towards necrosis.
Phytoreduced silver nanoparticles with polyphenols from Viburnum opulus L. fruit extract, administered during the embryological development of the male gonad, have testicular toxic effects in offspring even at 6 weeks after birth.
研究绿原酸提取的植物源银纳米粒子（AgNPs）对雄性仔鼠睾丸的影响。
在妊娠第 3-14 天，将两种不同剂量的 AgNPs（0.8 和 1.5mg/kg b.w.）和载体（PBS）给予 Wistar 雌性大鼠。在出生后 6 周，评估 AgNPs 对雄性仔鼠睾丸中与银含量相关的超微结构变化以及氧化应激、炎症和细胞死亡机制的参数。
妊娠期间给予的 AgNPs 穿过胎盘和睾丸屏障，并诱导氧化应激、DNA 损伤和自噬，作为细胞毒性的机制。AgNPs 暴露后炎症和细胞凋亡的标志物减少，而 NFkB 激活增加。TEM 检查显示 Sertoli 细胞的重要超微结构变化，大量空泡和细胞质变化提示细胞向坏死方向演变。
绿原酸提取的银纳米粒子，来自欧洲荚蒾果实提取物，具有多酚，在雄性性腺胚胎发育期间给药，即使在出生后 6 周，也会对后代睾丸产生毒性作用。
