Hashemi N, Mohaghegh M A, Hashemi M, Azami M, Mortazavidehkordi N, Hashemi C, Hejazi S H
Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Deputy of Research and Technology, North Khorasan University of Medical Sciences, Bojnurd, Iran.
Trop Biomed. 2016 Dec 1;33(4):689-696.
Antimony compounds are first line treatments for cutaneous leishmaniasis. The prognosis of the disease varies depending on the type of medicine and species. We aimed to determine the species responsible for cutaneous leishmaniasis in patients referred to Skin Diseases and Leishmaniasis Research Center in Isfahan and Bam Health Center (Kerman) in order to follow and assess the complete healing of the lesions. A total of 40 skin lesions samples were collected from patients with cutaneous leishmaniasis (CL) form January 2014 to 2015. Dermal scrapings were analyzed by examination of Giemsa-stained smears. Parasites were cultured and isolated in NNN and RPMI 1640 medium and DNA was extracted. We used PCR-RFLP assays of ITS1 genes for direct identification of Leishmania species. Treatment process was assessed after a treatment period with glucantime and healing of the studied cases was followed up. All the samples from Isfahan and Bam regions were L. major and L. tropica species respectively. In patients infected with L. major and L. tropica treated with glucantime, the shortest healing period was 40 days in 5(25%) and 60 days in 3(15.8%) patients, respectively and the longest healing period was 100 days in 1 (5%) and 160 days in 1 (5.3%) patient, respectively. The mean complete healing periods in patients with L. tropica and L. major were 100 and 58 days, respectively (P<0.001). Average recovery period for people with dry cutaneous leishmaniasis is longer than average recovery period for people with wet cutaeous leishmaniasis.
锑化合物是皮肤利什曼病的一线治疗药物。该疾病的预后因药物类型和物种而异。我们旨在确定转诊至伊斯法罕皮肤病和利什曼病研究中心以及克尔曼省巴姆健康中心的皮肤利什曼病患者中致病的物种，以便跟踪和评估病变的完全愈合情况。2014年1月至2015年，共收集了40例皮肤利什曼病（CL）患者的皮肤病变样本。通过检查吉姆萨染色涂片对皮肤刮片进行分析。将寄生虫在NNN和RPMI 1640培养基中培养和分离，并提取DNA。我们使用ITS1基因的PCR-RFLP分析直接鉴定利什曼原虫物种。在用葡糖胺进行治疗期后评估治疗过程，并对研究病例的愈合情况进行随访。来自伊斯法罕和巴姆地区的所有样本分别为大型利什曼原虫和热带利什曼原虫。在用葡糖胺治疗的感染大型利什曼原虫和热带利什曼原虫的患者中，最短愈合期分别为5例（25%）患者40天和3例（15.8%）患者60天，最长愈合期分别为1例（5%）患者100天和1例（5.3%）患者160天。热带利什曼原虫和大型利什曼原虫患者的平均完全愈合期分别为100天和58天（P<0.001）。干性皮肤利什曼病患者的平均恢复期长于湿性皮肤利什曼病患者的平均恢复期。
