Institut für Analytische Chemie, Universität Leipzig, Leipzig, Germany.
Department of Biotechnology & Enzyme Catalysis, Institute of Biochemistry, University of Greifswald, Greifswald, Germany; Junior Research Group Plastic Biodegradation, Institute of Biochemistry, University of Greifswald, Greifswald, Germany.
Methods Enzymol. 2021;648:231-252. doi: 10.1016/bs.mie.2020.11.002. Epub 2020 Dec 25.
The biocatalytic degradation of polyethylene terephthalate (PET) by thermophilic microbial enzymes has recently emerged as an option for a future eco-friendly recycling process for plastic waste, as it occurs under mild conditions and requires no harmful additives. In this chapter, we present a brief overview of solution and solid-state nuclear magnetic resonance (NMR) spectroscopic methods for the characterization of composition and chemical microstructure of PET and also associated chain dynamics over multiple time scales. Such detailed information provides an understanding of the enzymatic PET degradation mechanism by polyester hydrolases at the molecular level.
聚对苯二甲酸乙二醇酯（PET）的生物催化降解最近作为一种未来环保的塑料废物回收方法出现，因为它在温和的条件下进行，不需要有害的添加剂。在本章中，我们简要介绍了溶液和固态核磁共振（NMR）光谱方法在表征 PET 的组成和化学微观结构以及相关的链动力学多个时间尺度方面的应用。这种详细的信息提供了对聚酯水解酶在分子水平上催化 PET 降解机制的理解。
