检查人类和各种实验动物的红细胞聚集与血细胞比容之间的关系。

Examination of the relation between red blood cell aggregation and hematocrit in human and various experimental animals.

机构信息

Department of Operative Techniques and Surgical Research, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.

Doctoral School of Clinical Medicine, University of Debrecen, Debrecen, Hungary.

出版信息

Clin Hemorheol Microcirc. 2021;78(2):187-198. doi: 10.3233/CH-211109.

DOI:10.3233/CH-211109

PMID:33579832

Abstract

BACKGROUND

Red blood cell (RBC) aggregation plays an important role in the physiological processes of the microcirculation. The complete mechanism of aggregation is still unclear, and it is influenced by several cellular and plasmatic factors. One of these factors is the hematocrit (Hct).

OBJECTIVE

We hypothesized that the relation of RBC aggregation and Hct differs between species.

METHODS

From anticoagulated blood samples of healthy volunteers, rats, dogs, and pigs, 20, 40, and 60 %Hct RBC, autologous plasma suspensions were prepared. Hematological parameters and RBC aggregation was determined by light-transmission and light-reflection method.

RESULTS

Suspensions at 20%and 60%Hct expressed lower RBC aggregation than of 40%Hct suspensions, showing inter-species differences. By curve fitting the Hct at the highest aggregation value differed in species (human: 45.25%- M 5 s, 40.86%- amp; rat: 44.44 %- M1 10 s, 39.37%- amp; dog: 42.48%- M 5 s, 44.29%- amp; pig: 47.63%- M 5 s, 52.8%- amp).

CONCLUSION

RBC aggregation - hematocrit relation shows inter-species differences. Human blood was found to be the most sensitive for hematocrit changes. The more obvious differences could be detected by M 5 s by light-transmission method and amplitude parameter using light-reflection method.

摘要

背景

红细胞（RBC）聚集在微循环的生理过程中起着重要作用。聚集的完整机制仍不清楚，它受几种细胞和血浆因素的影响。其中一个因素是血细胞比容（Hct）。

目的

我们假设 RBC 聚集与 Hct 的关系在不同物种之间存在差异。

方法

从健康志愿者、大鼠、狗和猪的抗凝血液样本中，制备 20%、40%和 60%Hct RBC 、自体血浆悬浮液。通过透光和反射光法测定血液学参数和 RBC 聚集。

结果

20%和 60%Hct 的悬浮液表达的 RBC 聚集低于 40%Hct 悬浮液，表现出种间差异。通过曲线拟合，最高聚集值的 Hct 在不同物种中存在差异（人：45.25%-M5s，40.86%-amp；大鼠：44.44%-M1 10s，39.37%-amp；狗：42.48%-M5s，44.29%-amp；猪：47.63%-M5s，52.8%-amp）。