Dept. of Molecular Biology & Biochemistry, Simon Fraser University, Burnaby, British Columbia, V5A 1S6, Canada.
Dept. of Chemistry, Simon Fraser University, Burnaby, British Columbia, V5A 1S6, Canada.
Angew Chem Int Ed Engl. 2021 Apr 12;60(16):8722-8727. doi: 10.1002/anie.202016668. Epub 2021 Mar 10.
We report a new DNA nanostructure, an extended 1-dimensional composite built for the first time out of structurally robust yet conveniently disassembled DNA triple helices, interspersed with short stretches of G-quadruplexes. These "TQ Hybrid" 1-dimensional nanostructures require potassium ions and modestly acidic pH for their formation and are easily disassembled by changes to either of these requirements. We initially prepared and characterized a "monomeric" TQ Hybrid tile; followed by "sticky" TQs tiles, incorporating unique guanine-only sticky ends, that enable efficient self-assembly via G-quartet formation of nanostructures >150 nm in length, as seen with atomic force microscopy and transmission electron microscopy. We anticipate that such DNA TQ Hybrid structures will find unique and varied application as communication modules within larger nanostructures, and as sensors, logic gates, as well as in other aspects of DNA nanotechnology.
我们报告了一种新的 DNA 纳米结构，这是一种首次由结构稳定但方便拆开的 DNA 三螺旋结构，与短链 G-四链体交错而成的一维复合结构。这些“TQ 杂合体”一维纳米结构的形成需要钾离子和适度的酸性 pH 值，并且很容易通过改变这两个要求中的任何一个来拆开。我们最初制备并表征了一种“单体”TQ 杂合体瓷砖；随后是“粘性”TQs 瓷砖，它包含独特的仅含鸟嘌呤的粘性末端，通过 G-四联体的形成可以有效地进行自组装，形成长度超过 150nm 的纳米结构，这可以通过原子力显微镜和透射电子显微镜观察到。我们预计，这种 DNA TQ 杂合体结构将在更大的纳米结构内作为通信模块，以及作为传感器、逻辑门，以及在其他方面的 DNA 纳米技术中找到独特而多样的应用。
