对涂有导电膜的recA-DNA复合物进行扫描隧道显微镜观察。

Scanning tunneling microscopy of recA-DNA complexes coated with a conducting film.

作者信息

Amrein M, Stasiak A, Gross H, Stoll E, Travaglini G

机构信息

Institute for Cell Biology, Swiss Federal Institute of Technology ETH Hönggerberg, Zurich, Switzerland.

出版信息

Science. 1988 Apr 22;240(4851):514-6. doi: 10.1126/science.3358130.

DOI:10.1126/science.3358130

PMID:3358130

Abstract

A link between scanning tunneling microscopy (STM) and conventional transmission electron microscopy has been established for biological material by applying STM on freeze-dried recA-DNA complexes coated with a conducting film. The topography of the complexes observed by means of STM revealed a right-handed single helix composed of about six recA monomers per helical turn.

摘要

通过对涂有导电膜的冻干recA-DNA复合物应用扫描隧道显微镜（STM），已在生物材料中建立了扫描隧道显微镜与传统透射电子显微镜之间的联系。通过STM观察到的复合物的形貌显示出一种右手单螺旋结构，每螺旋圈由大约六个recA单体组成。