Wang Z H, Hartmann T, Baumeister W, Guckenberger R
Max-Planck-Institut für Biochemie, Abteilung Molekulare Strukturbiologie, Martinsried, Federal Republic of Germany.
Proc Natl Acad Sci U S A. 1990 Dec;87(23):9343-7. doi: 10.1073/pnas.87.23.9343.
A single-tube scanning tunneling microscope has been zeta-calibrated by using atomic steps of crystalline gold and was used for measuring the thickness of two biological samples, metal-coated as well as uncoated. The hexagonal surface layer of the bacterium Deinococcus radiodurans with an open network-type structure shows thickness values that are strongly influenced by the substrate and the preparation method. In contrast, the thickness of the purple membrane of Halobacterium halobium with its densely packed less-corrugated structure exhibits very little variation in thickness in coated preparations and the values obtained are in good agreement with x-ray data.
一台单管扫描隧道显微镜已通过使用结晶金的原子台阶进行了ζ校准，并用于测量两个生物样品的厚度，这两个样品既有金属涂层的，也有未涂层的。具有开放网络型结构的耐辐射球菌的六边形表面层显示出的厚度值受到底物和制备方法的强烈影响。相比之下，具有紧密堆积且波纹较少结构的嗜盐菌紫膜在有涂层的制剂中厚度变化很小，所获得的值与X射线数据吻合良好。
