Univ. Grenoble Alpes, CNRS, Grenoble INP (Institute of Engineering Univ. Grenoble Alpes), LGP2, F-38000 Grenoble, France; Arjowiggins France SAS, Voiron F-38500, France.
Univ. Grenoble Alpes, CNRS, Grenoble INP (Institute of Engineering Univ. Grenoble Alpes), LGP2, F-38000 Grenoble, France.
Bioresour Technol. 2021 May;327:124819. doi: 10.1016/j.biortech.2021.124819. Epub 2021 Feb 5.
The aim of this work was to study the cellulose fibers deconstruction by twin-screw extrusion with in situ enzymatic hydrolysis via bioextrusion, for cellulose nanofibrils (CNF) production. Cellulose pulp was extruded with an optimized screw profile, with or without (reference) the addition of an enzymatic solution. An increase of crystallinity index from 67.0% to 73.7% and decrease of DP from 1003 to 419 were observed with bioextrusion. Direct activity measurements of the enzyme confirmed its activity during the process (sugar content increasing from 0.07 ± 0.004 to 2.38 ± 0.003 mg/mL) and after the process (specific activities around 0.20 CMCU/mL). Enzymes were not deactivated during bioextrusion and could be recycled. CNF properties were higher with bioextrusion compared to reference (respective quality indices of 55.5 ± 2.7 and 39.8 ± 2.8), with a lower energy consumption. This proof of concept could be optimized for the industrial production of highly concentrated CNF.
这项工作的目的是通过生物挤出，用双螺杆挤压对纤维素纤维进行原位酶水解解聚，以生产纤维素纳米纤维（CNF）。纤维素纸浆在优化的螺杆轮廓下进行挤压，无论是否添加酶溶液（参考）。通过生物挤出，结晶度指数从 67.0%增加到 73.7%，DP 从 1003 降低到 419。直接对酶进行活性测量证实了其在该过程中的活性（糖含量从 0.07±0.004 增加到 2.38±0.003 mg/mL）以及该过程之后的活性（约 0.20 CMCU/mL 的比酶活）。酶在生物挤出过程中没有失活，可以回收利用。与参考（分别为 55.5±2.7 和 39.8±2.8）相比，生物挤出的 CNF 性能更高，能量消耗更低。这一概念验证可以优化高浓度 CNF 的工业生产。
