Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Department of Obstetrics and Gynaecology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Anaerobe. 2021 Jun;69:102343. doi: 10.1016/j.anaerobe.2021.102343. Epub 2021 Feb 11.
The present study aimed to determine the utility of detection of co-infection of Gardnerella vaginalis and Atopobium vaginae using qualitative PCR for diagnosing bacterial vaginosis (BV). Vaginal samples (n = 385) categorized as positive (n = 108) or negative (n = 208) for bacterial vaginosis based on the Nugent scoring system, were analyzed for the presence of G. vaginalis and A. vaginae by conventional PCR. We compared the sensitivity, specificity, positive predictive value, negative predictive value and odds ratio for the detection of each bacterium alone with the combination of the two bacteria for diagnosing BV. The detection of co-infection of the two bacteria demonstrated a sensitivity of 96%, a specificity of 82.9%, a positive predictive value of 68.5%, a negative predictive value of 98.2% with an odds ratio of 116 (CI -32 - 409). In our study, we found a high sensitivity, specificity, negative predictive value and odds ratio for the detection of co-infection of A. vaginae and G. vaginalis for the diagnosis of BV.
本研究旨在确定使用定性 PCR 检测阴道加德纳菌和阴道阿托波菌的合并感染用于诊断细菌性阴道病（BV）的效用。根据 Nugent 评分系统，将阴道样本（n=385）分为阳性（n=108）或阴性（n=208），通过常规 PCR 分析阴道加德纳菌和阴道阿托波菌的存在情况。我们比较了单独检测每种细菌与联合检测两种细菌对诊断 BV 的敏感性、特异性、阳性预测值、阴性预测值和优势比。两种细菌合并感染的检测显示出 96%的敏感性、82.9%的特异性、68.5%的阳性预测值、98.2%的阴性预测值和 116 的优势比（CI-32-409）。在我们的研究中，我们发现检测阴道阿托波菌和阴道加德纳菌合并感染用于诊断 BV 的检测具有较高的敏感性、特异性、阴性预测值和优势比。
